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MilliporeSigma

53516-U

Ascentis® Express 90 Å RP-Amide (2.7 μm) HPLC Columns

L × I.D. 5 mm × 3 mm, HPLC Guard Cartridge, pkg of 3 ea

Synonym(s):

Core-shell (SPP) Fused Core polar embedded HPLC column

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3 EA

$713.00

$713.00

Estimated to ship onDecember 15, 2025Details

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

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Product Name

Ascentis® Express RP-Amide, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea

material

stainless steel column

Quality Level

100

agency

suitable for USP L60

product line

Ascentis®

feature

endcapped

packaging

pkg of 3 ea

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

5 mm × 3 mm

surface area

135 m2/g

matrix

Fused-Core® particle platform
superficially porous particle

matrix active group

amide, alkyl phase

particle size

2.7 μm

pore size

90 Å

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

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53514-U53519-U53599-U
Ascentis® Express RP-Amide, 2.7 μm Guard Cartridge 2.7 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea

Supelco

53516-U

Ascentis® Express RP-Amide, 2.7 μm Guard Cartridge

Ascentis® Express RP-Amide, 2.7 μm Guard Cartridge 2.7 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea

Supelco

53514-U

Ascentis® Express RP-Amide, 2.7 μm Guard Cartridge

Ascentis® Express RP-Amide, 2.7 μm Guard Cartridge 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

Supelco

53519-U

Ascentis® Express RP-Amide, 2.7 μm Guard Cartridge

Ascentis® Express F5, 2.7 μm Guard Cartridge 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

Supelco

53599-U

Ascentis® Express F5, 2.7 μm Guard Cartridge

L × I.D.

5 mm × 3 mm

L × I.D.

5 mm × 2.1 mm

L × I.D.

5 mm × 4.6 mm

L × I.D.

5 mm × 4.6 mm

particle size

2.7 μm

particle size

2.7 μm

particle size

2.7 μm

particle size

2.7 μm

matrix active group

amide, alkyl phase

matrix active group

amide, alkyl phase

matrix active group

amide, alkyl phase

matrix active group

PFP (pentafluorophenyl) phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

pore size

90 Å

pore size

90 Å

pore size

90 Å

pore size

90 Å

matrix

Fused-Core® particle platform, superficially porous particle

matrix

Fused-Core® particle platform, superficially porous particle

matrix

Fused-Core® particle platform, superficially porous particle

matrix

superficially porous particle

General description

Ascentis Express Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of Ascentis Express columns. These Ascentis Express guard columns are capable of continuous use at pressures up to 9000 psi (600 bar) with only hand-tightening. Guard cartridges are easily replaced without removing the guard column holder from the flow path. The cartridges are packed with Ascentis Express Fused-Core® particles. Order guard column holder separately.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Michael T Furlong et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 926, 36-41 (2013-04-02)
Clopidogrel is prescribed for the treatment of Acute Coronary Syndrome and recent myocardial infarction, recent stroke, or established peripheral arterial disease. A sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay was developed and validated to enable reliable
S Fan et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 30(12), 2093-2101 (2013-10-22)
A liquid chromatography-linear ion-trap spectrometry (LC-MS³) method using β-receptor molecular-imprinted polymer (MIP) solid-phase extraction (SPE) as clean-up was developed to determine simultaneously and confirmatively residues of 25 β₂-agonists and 21 β-blockers in urine samples. Urine samples were subjected to enzymatic
Hirotaka Kushida et al.
Biomedical chromatography : BMC, 27(12), 1647-1656 (2013-07-03)
Uncaria Hook (UH) alkaloids are involved in the beneficial effects of Yokukansan. However, the pharmacokinetics of UH alkaloids after oral administration of Yokukansan has not yet been sufficiently investigated. Therefore, we developed and validated a sensitive and specific high-performance liquid
Martin Mzik et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1020, 85-89 (2016-04-01)
A simple, rapid and sensitive method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated for the quantitative determination in rat plasma of a new candidate for AD treatment, namely PC 48 (a 7-MEOTA-donepezil like compound) in
Erzsébet Oláh et al.
Journal of chromatography. A, 1217(23), 3642-3653 (2010-04-23)
Today sub-2 microm packed columns are very popular to conduct fast chromatographic separations. The mass-transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass-transfer resistance. Another approach applies
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Ascentis® Express RP-Amide Columns for HPLC and UHPLC

Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.

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