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53581-U
Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns
L × I.D. 3 cm × 4.6 mm, HPLC Column
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 ea | Check Cart for Availability | $566.00 $424.50 |
About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
3 cm × 4.6 mm
Particle size:
2.7 μm
Matrix active group:
PFP (pentafluorophenyl) phase
Pore size:
90 Å pore size
Matrix:
Fused-Core particle platform, superficially porous particle
$424.50
List Price$566.00Save 25%Web-Only Promotion
Check Cart for Availability
Product Name
Ascentis® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 3 cm × 4.6 mm
material
stainless steel column
Quality Level
agency
suitable for USP L43
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range, 600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
L × I.D.
3 cm × 4.6 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform, superficially porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
2.7 μm
pore size
90 Å pore size
operating pH
2-8
application(s)
food and beverages
separation technique
reversed phase
General description
The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
This Item | |||
|---|---|---|---|
| matrix active group PFP (pentafluorophenyl) phase | matrix active group PFP (pentafluorophenyl) phase | matrix active group PFP (pentafluorophenyl) phase | matrix active group PFP (pentafluorophenyl) phase |
| L × I.D. 3 cm × 4.6 mm | L × I.D. 15 cm × 3 mm | L × I.D. 10 cm × 3 mm | L × I.D. 5 cm × 2.1 mm |
| separation technique reversed phase | separation technique reversed phase | separation technique reversed phase | separation technique reversed phase |
| particle size 2.7 μm | particle size 2.7 μm | particle size 2.7 μm | particle size 2.7 μm |
| technique(s) HPLC: suitable, UHPLC-MS: suitable, LC/MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable |
| matrix Fused-Core particle platform, superficially porous particle | matrix Fused-Core particle platform, superficially porous particle | matrix Fused-Core particle platform, superficially porous particle | matrix Fused-Core particle platform, superficially porous particle |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®
Articles
Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds
Related Content
Ascentis® Express 2.7 μm F5 Column Care & Use Sheet
Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 53581-U | 04061838531629 |
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What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
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Can I use Ascentis® Express F5 HPLC Columns on any type of HPLC system?
1 answer-
Ascentis® Express F5 HPLC Columns HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. These columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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Do I need special fittings and tubing to connect Ascentis® Express F5 HPLC Columns?
1 answer-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
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Can I use Ascentis® Express F5 HPLC Columns on a UHPLC system?
1 answer-
Ascentis® Express F5 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis® Express F5 HPLC Columns outperform sub-2 μm micron columns on many applications since Ascentis® Express F5 HPLC Columns provide the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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Are guard columns available for Ascentis® Express F5 4.6 mm ID HPLC Columns?
1 answer-
Yes, we have guard columns available for Ascentis® Express F5 4.6 mm ID HPLC Column. Product 53500-U is the Ascentis® Express Guard Holder and Product 53599-U Ascentis® Express F5 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 4.6 mm .
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Is there anything special I need to do to my HPLC system to use Ascentis® Express F5 HPLC Columns?
1 answer-
Nothing special is required to use Ascentis® Express F5 HPLC Columns. To obtain the full benefits of Ascentis® Express Columns, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
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What is the carbon load for Ascentis® Express F5 HPLC Columns?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express F5 HPLC columns is 3.5 micromoles/meter-squared.
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Can Ascentis® Express F5 HPLC columns be used for LC-MS?
1 answer-
Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis® Express F5 HPLC Columns for high throughput UHPLC and LC-MS are that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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What is the pressure rating for Ascentis® Express F5 HPLC Columns?
1 answer-
Ascentis® Express F5 HPLC Columns are stable to operating pressures up to 600 bar (9000 psi).
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How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 answer-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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