53590-U

Supelco

Ascentis® Express F5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 4.6 mm

eCl@ss:
32110501
NACRES:
SB.52
Pricing and availability is not currently available.

Quality Level

Agency/Method

suitable for USP L43

feature

endcapped

mfr. no.

Ascentis

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

application(s)

HPLC: suitable
UHPLC: suitable

L × I.D.

10 cm × 4.6 mm

impurities

<5 ppm metals

matrix

Fused-Core particle platform

matrix active group

PFP (pentafluorophenyl) phase

particle size

2.7 μm

pore size

90 Å pore size

operating pH range

1 - 9

Featured Industry

Food and Beverages

separation technique

hydrophilic interaction (HILIC)
reversed phase

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Related Categories

General description

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

WGK Germany

3

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is special about Ascentis Express?

    Ascentis Express columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis Express provides the benefits of sub-2 μm particles but at much lower backpressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. When are additional phases expected?

    Additional phases are being developed.  The best way to track new products is to visit Ascentis Express for the latest updates.

  7. How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?

    The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

  8. What is special about Ascentis® Express F5 HPLC Columns?

    Ascentis® Express F5 HPLC Columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis® Express columns provide the benefits of sub-2 μm particles but at much lower backpressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

  9. Are guard columns available for Ascentis® Express F5 4.6 mm ID HPLC Columns?

    Yes, we have guard columns available for Ascentis® Express F5 4.6 mm ID HPLC Column.  Product 53500-U is the Ascentis® Express Guard Holder and Product 53599-U Ascentis® Express F5 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 4.6 mm .

  10. Can I use Ascentis® Express F5 HPLC Columns on any type of HPLC system?

    Ascentis® Express F5 HPLC Columns HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  These columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

Articles
Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds
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Protocols
RP-Amide and HILIC, both amenable to the analysis of polar compounds, were successfully used to resolve several beverage components, including sugars, vitamins, sweeteners, preservatives, and caffeine.
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Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®
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