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MilliporeSigma

53811-U

Ascentis® Express C18 (2.7 μm) HPLC Column

L × I.D. 5 cm × 3 mm, pk of 1 ea

Synonym(s):

Core-shell (SPP) Fused Core C18 HPLC column

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1 EA

$809.00

$809.00

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
eCl@ss:
32110501
L × i.d.:
5 cm × 3 mm
Particle size:
2.7 μm
Matrix active group:
C18 (octadecyl) phase
Pore size:
90 Å
Matrix:
Fused-Core particle platform, superficially porous particle

Key Documents

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material

stainless steel column

agency

suitable for USP L1

product line

Ascentis®

feature

endcapped

packaging

pk of 1 ea

manufacturer/tradename

Ascentis®

parameter

60 °C temp. range, 600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

5 cm × 3 mm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

2.7 μm

pore size

90 Å

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

Quality Level

100

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This Item
582711-U53829-U50630-U
Ascentis&#174; Express C18 (2.7 &#956;m) HPLC Column L × I.D. 5&#160;cm × 3&#160;mm, pk of 1&#160;ea

Supelco

53811-U

Ascentis® Express C18 (2.7 μm) HPLC Column

Ascentis&#174; Express C18 (2.7 &#956;m) HPLC Column L × I.D. 5&#160;cm × 1&#160;mm, pk of 1&#160;ea

Supelco

582711-U

Ascentis® Express C18 (2.7 μm) HPLC Column

Ascentis&#174; Express C18 (2.7 &#956;m) HPLC Column L × I.D. 15&#160;cm × 4.6&#160;mm, pk of 1&#160;ea

Supelco

53829-U

Ascentis® Express C18 (2.7 μm) HPLC Column

Ascentis&#174; Express C18 (2.7 &#956;m) HPLC Column L × I.D. 10&#160;cm × 1.5&#160;mm, pk of 1&#160;ea

Supelco

50630-U

Ascentis® Express C18 (2.7 μm) HPLC Column

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

-

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

L × I.D.

5 cm × 3 mm

L × I.D.

5 cm × 1 mm

L × I.D.

15 cm × 4.6 mm

L × I.D.

10 cm × 1.5 mm

particle size

2.7 μm

particle size

2.7 μm

particle size

2.7 μm

particle size

std dev 2.7 μm

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform

General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Application

HPLC Analysis of Capsaicins in Chili Powder on Ascentis® Express C18, 2.7 μm
UHPLC/MS Analysis of Corn Oil on Ascentis® Express C30 Compared to Ascentis® Express C18
UHPLC Analysis of Paracetamol and Impurities on Ascentis® Express C18, 2.7 μm
HPLC Analysis of Parabens on Ascentis®Express C18, 2.7 μm

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

pcodes

P280

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
USGR005134
USGR005133
USGR005132
USGR005131
USGR005130

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Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
Petra Šilarová et al.
Food chemistry, 237, 471-480 (2017-08-03)
The degradation of catechins and other phenolics in green tea infusions were monitored using fast HPLC/MS separation. The final separation was performed within 2.5min using Ascentis Express C18 column (50mm×2.1mm i.d.) packed with 2μm porous shell particles. Degradation was studied
Judy Stone
Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)
Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %
Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within
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Derivatization and Separation of Aliphatic Amines

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

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