While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
53858-U
Ascentis® Express 90Å C8 (2.7 μm) HPLC Columns
L × I.D. 7.5 cm × 4.6 mm, HPLC Column
Synonym(s):
Core-shell (SPP) Fused Core C8 HPLC column
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| Size/SKU | Availability | Price |
|---|---|---|
1 ea | Available to ship TODAYfromMILWAUKEE | $861.00 $645.75 |
About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
7.5 cm × 4.6 mm
Particle size:
2.7 μm
Matrix active group:
C8 (RP8), C8 (octyl) phase
Pore size:
90 Å
Matrix:
Fused-Core particle platform, superficially porous particle
$645.75
List Price$861.00Save 25%Web-Only Promotion
Available to ship TODAYDetails
Product Name
Ascentis® Express C8, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 7.5 cm × 4.6 mm
material
stainless steel column
Quality Segment
agency
suitable for USP L7
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range, 600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
L × I.D.
7.5 cm × 4.6 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform, superficially porous particle
matrix active group
C8 (RP8), C8 (octyl) phase
particle size
2.7 μm
pore size
90 Å
operating pH range
2-9
application(s)
food and beverages
separation technique
reversed phase
General description
Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.
Visit the Ascentis Express home page for more information on this new column technology.
Application
- Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review explores the evolution and performance of superficially porous silica columns in high-performance liquid chromatography (HPLC), including those similar to the Ascentis Express C8, 2.7 μm. The focus is on the technology′s impact on enhancing analytical speed and efficiency (Ali et al., 2012).
- Development and validation of an HPLC-MS/MS method to determine clopidogrel in human plasma. Use of incurred samples to test back-conversion.: This study details the development of an HPLC-MS/MS analytical method using columns such as the Ascentis Express C8, 2.7 μm, aimed at quantifying clopidogrel in plasma, demonstrating the column′s utility in clinical pharmacokinetic assessments (Silvestro et al., 2010).
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters
1 of 1
This Item | |||
|---|---|---|---|
| description 2.7 μm particle size, L × I.D. 7.5 cm × 4.6 mm | description 2.7 μm particle size, L × I.D. 3 cm × 3 mm | description 2.7 μm particle size, L × I.D. 7.5 cm × 2.1 mm | description 2.7 μm particle size, L × I.D. 5 cm × 2.1 mm |
| particle size 2.7 μm | particle size 2.7 μm | particle size 2.7 μm | particle size 2.7 μm |
| separation technique reversed phase | separation technique reversed phase | separation technique reversed phase | separation technique reversed phase |
| L × I.D. 7.5 cm × 4.6 mm | L × I.D. 3 cm × 3 mm | L × I.D. 7.5 cm × 2.1 mm | L × I.D. 5 cm × 2.1 mm |
| matrix active group C8 (RP8), C8 (octyl) phase | matrix active group C8 (RP8), C8 (octyl) phase | matrix active group C8 (RP8), C8 (octyl) phase | matrix active group C8 (RP8), C8 (octyl) phase |
| product line Ascentis® | product line Ascentis® | product line Ascentis® | product line Ascentis® |
| technique(s) HPLC: suitable, UHPLC-MS: suitable, LC/MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Find documentation for the products that you have recently purchased in the Document Library.
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Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
1 answer-
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What flow rate should I use with Ascentis® Express HPLC Columns?
1 answer-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
1 answer-
Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
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Can Ascentis® Express HPLC Columns be used for LC-MS?
1 answer-
Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 answer-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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Can I use Ascentis Express on any type of HPLC system?
1 answer-
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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Can I use Ascentis Express on a UHPLC system?
1 answer-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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