The column housing for the Ascentis Express, 300 um x 5 cm (53992-U) is made of PEEK with stainless steel end fittings.
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| Size/SKU | Availability | Price |
|---|---|---|
1 ea | Check Cart for Availability | $1,280.00 |
About This Item
$1,280.00
Product Name
Ascentis® Express C18, 2.7 Micron Capillary HPLC Column, 2.7 μm particle size, L × I.D. 5 cm × 300 μm
material
stainless steel column
Quality Segment
agency
suitable for USP L1
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
400 bar pressure (5801 psi), 60 °C temp. range
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
L × I.D.
5 cm × 300 μm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform, superficially porous particle
matrix active group
C18 (RP18), C18 (octadecyl) phase
particle size
2.7 μm
pore size
90 Å pore size
operating pH
2-9
application(s)
food and beverages
separation technique
reversed phase
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the column housing material for the Ascentis Express, 300 um x 5 cm (53992-U) capillary LC column? Is it a stainless steel column housing, or is it made of another material such as PEEK or fused silica? Additionally, if it is steel, are there other capillary columns available with metal-free housings?
1 answer-
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Can I use Ascentis Express on a UHPLC system?
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Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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What is the pressure rating for 300 μm I.D. Ascentis® Express Capillary HPLC columns?
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The 300 μm I.D. Ascentis® Express Capillary HPLC columns are stable to operating pressures up to 6500 psi.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the carbon load for Ascentis® Express C18 HPLC Columns?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared
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