Mono S® 5/50 GL

10 μm particle size, L × I.D. 5 cm × 5 mm

Amersham Biosciences Ion Exchange Columns

L × I.D.

5 cm × 5 mm



matrix active group

sulfonic acid

particle size

10 μm


mode of use ion exchange

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General description

Mono columns are highly efficient, pH-stable columns designed for high performance ion exchange separations of proteins, peptides, and polynucleotides. The unique properties of these columns are based on MonoBeads support, a beaded hydrophilic material with the narrowest particle size distribution of any chromatographic support. This monodispersity permits high flow rates at relatively low backpressures.


Mono columns are used in applications including peptide mapping and monoclonal antibody purification. Mono S columns have been used in cancer research studies.

Legal Information

Mono S is a registered trademark of Cytiva

Certificate of Analysis

Certificate of Origin

Xiu-Juan Ye et al.
Journal of agricultural and food chemistry, 59(18), 10232-10238 (2011-08-13)
A 30 kDa antifungal protein was purified from red cabbage ( Brassica oleracea ) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange...
Evandro Fei Fang et al.
Archives of toxicology, 86(2), 293-304 (2011-10-05)
A 20-kDa Kunitz-type trypsin-chymotrypsin inhibitor, Bauhinia purpurea trypsin inhibitor (BPLTI), has been isolated from the seeds of B. purpurea L. by using liquid chromatography procedures that involved ion exchange chromatography on Sp-Sepharose and Mono S and gel filtration on Superdex...

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