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Supelco

96-Well Protein Precipitation Filter Plate

pk of 1

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NACRES:
NB.21

packaging

pk of 1

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technique(s)

solid phase extraction (SPE): suitable

volume

2 mL

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technique(s)

solid phase extraction (SPE): suitable

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technique(s)

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volume

2 mL

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General description

The 96-well protein precipitation filter plate is ideal for removing precipitated proteins from biological plasma/serum. The plate consists of a 0.2 μm hydrophobic graded filter/frit. Biological plasma is first added to the 96-well plate followed by a protein precipitating agent (e.g., acetonitrile). After a brief mixing step, vacuum is applied to the plate, and the filter/frit removes precipitated proteins from the sample. The resulting filtrate is ready for further processing and/or analysis.

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Articles

We are presenting an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.

This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

Protocols

A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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