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Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 5 cm × 4.6 mm



stainless steel column


suitable for USP L60

product line







1 ea of

extent of labeling

19.5% Carbon loading


≤70 °C temp. range
400 bar pressure (5801 psi)


HPLC: suitable
LC/MS: suitable

L × I.D.

5 cm × 4.6 mm

surface area

450 m2/g

surface coverage

2.7 μmol/m2


<5 ppm metals


fully porous particle
silica gel high purity, spherical

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

100 Å

operating pH range



food and beverages

separation technique

reversed phase

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General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Features and Benefits

  • Excellent retention and peak shape for polar compounds
  • 100% aqueous compatibility
  • Ultra low bleed, LC-MS compatible
  • Unique selectivity

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

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Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express


HPLC Separation of Fluorinated Pharmaceutical Compounds on Supelco Ascentis Columns

Fluorinated compounds possess distinct characteristics that confer desirable physico-chemical properties compared to their non-fl uorinated analogs. Their abundance in pharmaceuticals, chemicals, consumer products and as environmental pollutants dictates the need for a reliable analytical method. Ascentis HPLC columns, by virtue of their effi ciency and choices in stationary phase selectivity, are ideally suited for the analysis of fl uorinated compounds.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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