567209-U

Supelco

Discovery® BIO Wide Pore C18 HPLC Column

10 μm particle size, L × I.D. 25 cm × 10 mm

eCl@ss:
32110501
NACRES:
SB.52
Pricing and availability is not currently available.

Quality Level

Agency/Method

suitable for USP L1

feature

endcapped

mfr. no.

Discovery

extent of labeling

9.2% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

application(s)

HPLC: suitable

L × I.D.

25 cm × 10 mm

surface coverage

3.6 μmol/m2

matrix

silica particle platform

matrix active group

C18 (octadecyl) phase

particle size

10 μm

pore size

300 Å

surface area

100 m2/g

separation technique

reversed phase

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General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC
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DiscoveryBIO Wide Pore C18 products offered by Sigma-Aldrich.
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