MilliporeSigma
All Photos(1)

567211-U

Supelco

Discovery® BIO Wide Pore C18 HPLC Column

10 μm particle size, L × I.D. 15 cm × 21.2 mm

eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

Quality Level

Agency

suitable for USP L1

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

9.2% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 21.2 mm

surface coverage

3.6 μmol/m2

matrix

silica particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

10 μm

pore size

300 Å

surface area

100 m2/g

operating pH

2-8

separation technique

reversed phase

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service