Discovery® BIO Wide Pore C8 HPLC Column

10 μm particle size, L × I.D. 25 cm × 10 mm

Pricing and availability is not currently available.

Quality Level


suitable for USP L7



mfr. no.


extent of labeling

5% Carbon loading


0-70 °C temperature
400 bar pressure (5801 psi)


HPLC: suitable

L × I.D.

25 cm × 10 mm

surface coverage

4 μmol/m2


silica particle platform

matrix active group

C8 (octyl) phase

particle size

10 μm

pore size

300 Å

surface area

100 m2/g

separation technique

reversed phase

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General description

Discovery BIO Wide Pore C8 exhibits hydrophobicity intermediate between the Discovery BIO Wide Pore C5 and the Discovery BIO Wide Pore C18. The difference in hydrophobicity gives it unique selectivity relative to these other phases. It is ideal for peptide mapping because it provides complementary information compared to a C18 separation. Because of its intermediate hydrophobicity, we also recommend it for method development or scouting work. As with all Discovery BIO Wide Pore phases, the C8 phase gives efficient, symmetrical peaks, exceptional stability, long column lifetime, and LC-MS compatibility.

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

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