568222-U
Discovery® BIO Wide Pore C18 (5 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm
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product name
Discovery® BIO Wide Pore C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Quality Level
agency
suitable for USP L1
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
9.2% Carbon loading
parameter
0-70 °C temperature
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
15 cm × 4.6 mm
surface area
100 m2/g
surface coverage
3.6 μmol/m2
matrix
silica particle platform
fully porous particle
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
300 Å
operating pH
2-8
separation technique
reversed phase
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General description
Application
- Kinetic performance comparison of superficially porous, fully porous and monolithic reversed-phase columns by gradient kinetic plots for the separation of protein biopharmaceuticals.: This study provides a detailed kinetic performance comparison of different types of reversed-phase HPLC columns, including the Discovery® BIO Wide Pore C18 HPLC Column. The research highlights its efficiency and resolution in separating complex protein biopharmaceuticals, making it a valuable tool for analytical chemists in biopharmaceutical analysis (Jaag et al., 2022).
- RP-LC x RP-LC analysis of a tryptic digest using a combination of totally porous and partially porous stationary phases.: This paper investigates the use of reverse-phase liquid chromatography (RP-LC) with both totally porous and partially porous stationary phases, including the BIO Wide Pore C18 HPLC Column, to analyze tryptic digests. The findings demonstrate its robustness and high resolution in proteomics applications, enabling precise peptide mapping and protein characterization (Mondello et al., 2010).
Legal Information
Antibody
Application
Guard cartridge
Certificates of Analysis (COA)
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Articles
Supelco Preparative HPLC products for Pharmaceutical Development and Production
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