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568430-U

Supelco

Discovery® BIO Wide Pore C5 HPLC Column

5 μm particle size, L × I.D. 25 cm × 10 mm

eCl@ss:
32110501
NACRES:
SB.52
Pricing and availability is not currently available.

Quality Level

feature

endcapped

mfr. no.

Discovery

extent of labeling

3.5% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

application(s)

HPLC: suitable

L × I.D.

25 cm × 10 mm

surface coverage

4.5 μmol/m2

matrix

silica particle platform

matrix active group

C5 (pentyl) phase

particle size

5 μm

pore size

300 Å

surface area

100 m2/g

separation technique

reversed phase

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General description

Discovery BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC. Long-chain phases, like C8 or C18, are often too hydrophobic for proteins and can cause excessively long retention time or even irreversible binding to the column. For this reason short-chain phases, typically C3 or C4, are often used for RP-HPLC of proteins. However, these short-chain phases are susceptible to hydrolysis, resulting in short column lifetime, especially at low pH. The Discovery BIO Wide Pore C5 gives elution order similar to a conventional C4, yet has enhanced pH stability for longer column lifetime. Generally, higher efficiency separations are achievable on the Discovery BIO Wide Pore C5 because of the improvements we have made to the silica and bonded-phase chemistry.

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC
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Discovery BIO Wide Pore C5 products offered.
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