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MilliporeSigma

575659-U

HybridSPE®-PLus 96-Well solid phase extraction (SPE) Plate

Volume 2 mL, bed wt., 50 mg, pk of 1 ea

Synonym(s):

HybridSPE®-PLus

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UNSPSC Code:
41104018
NACRES:
NB.21

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Product Name

HybridSPE®-PLus 96-Well Plate, volume 2 mL, pk of 1 ea

description

96-well Plate

product line

HybridSPE®

composition

bed wt., 50 mg

packaging

pk of 1 ea

technique(s)

solid phase extraction (SPE): suitable

volume

2 mL

matrix active group

zirconia-based phase

Quality Level

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This Item
52813-U55269-U52797-U
matrix active group

zirconia-based phase

matrix active group

zirconia-based phase

matrix active group

zirconia-based phase

matrix active group

zirconia-based phase

technique(s)

solid phase extraction (SPE): suitable

technique(s)

solid phase extraction (SPE): suitable

technique(s)

solid phase extraction (SPE): suitable

technique(s)

solid phase extraction (SPE): suitable

composition

bed wt., 50 mg

composition

-

composition

bed wt., 30 mg

composition

Bed wt., 100 mg

packaging

pk of 1 ea

packaging

-

packaging

pk of 100

packaging

pk of 54 ×

description

96-well Plate

description

-

description

-

description

Cartridge

product line

HybridSPE®

product line

HybridSPE®

product line

-

product line

-

Features and Benefits

  • Simple 2-3 step standard methodology analogous to traditional protein precipitation provides simultaneous removal of both proteins and phospholipids
  • Unique Lewis acid/base retention mechanism effectively removes phospholipids from both hydrophilic and hydrophobic analytes of interest (unlike competitive chemistries) eliminating phospholipid-induced ion suppression
  • Highly reproducible, consistent product performance
  • Provides cleaner samples for longer column life and less instrument downtime
  • Available in 96-well and 1 mL cartridge dimensions

General description

HybridSPE-PLus is the next generation of HybridSPE-Phospholipid technology. The first of its kind, HybridSPE-Phospholipid technology was first introduced in 2008. It fuses the simple, standardized methodology of traditional protein precipitation with the specificity of solid phase extraction (SPE) for the simultaneous removal of proteins and phospholipids from biological samples prior to LC-MS analysis. Unlike other phospholipid removal products that use a hydrophobic retention mechanism to remove phsopholipids from biological samples, HybridSPE-Phospholipid technology uses a unique Lewis acid/base retention mechanism that allows it to separate phospholipids from even very hydrophobic analytes, such as vitamins, which are often retained along with phospholipids on competitive products.

Our HybridSPE-PLus products offer the same time-proven chemistry of our traditional HybridSPE-Phospholipid products coupled with manufacturing enhancements that stem from years of production experience. These manufacturing enhancements have allowed us to increase flow consistency and reduce sample hold up volumes, further improving analyte recoveries and assay reproducibility. We invite customers to take advantage of the newest technology; HybridSPE-PLus.

Legal Information

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

Using HybridSPE-Phospholipid, Ascentis Express, and Other LC-MS Workflow Components

Protocols

LC/MS/MS Analysis of Interacting Cardiac Drugs Digoxin, Quinidine, Amiodarone and Verapamil on Titan™ C18

Immunoassay-based methods for digitoxin or digoxin exist, but are time consuming, labor intensive, and not selective due to similarities in their chemical structures.

Related Content

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Las moléculas pequeñas son iones y compuestos con un peso molecular inferior a 900 daltons. Estos compuestos pueden separarse y analizarse eficazmente mediante HPLC, UHPLC y LC-MS utilizando principalmente partículas de sílice o fases estacionarias monolíticas con una amplia gama de composiciones químicas de columna (modificaciones).

Solid phase extraction purifies samples for chromatographic analysis, improving data quality and protecting analytical columns.

La extracción en fase sólida purifica las muestras para el análisis cromatográfico, mejorando la calidad de los datos y protegiendo las columnas analíticas.

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