577425-U

BIOshell^™ IgG 1000 Å Diphenyl (2.7 μm) HPLC Columns

L × I.D. 10 cm × 3 mm, HPLC Column

• Synonym(s): BIOshell^™ IgG 1000 Å Diphenyl, 2.7 μm HPLC Column
• UNSPSC Code: 41115700
• NACRES: SB.52
• Particle size: 2.7 μm
• Matrix active group: bonding phase, Diphenyl (diphenylmethyl)
• Pore size: 1000 Å pore size
• Matrix: Fused-Core^® particle platform, superficially porous particle

Properties

• Product Name: BIOshell^™ IgG 1000 Å Diphenyl, 2.7 μm HPLC Column, column L × I.D. 10 cm × 3 mm
• material: stainless steel column
• agency: suitable for USP L11
• product line: BIOshell^™
• mol wt: 2000 - 1,000,000
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 1.0% loading
• technique(s): HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
• column L × I.D.: 10 cm × 3 mm
• surface area: 22 m²/g
• matrix: Fused-Core^® particle platform, superficially porous particle
• matrix active group: bonding phase, Diphenyl (diphenylmethyl)
• particle size: 2.7 μm
• pore size: 1000 Å pore size
• operating pH range: 2-9
• separation technique: reversed phase

Description

General description

BIOshell^™ IgG 1000 Å Diphenyl is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000 Å) Fused-Core^® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thin porous shell and the small overall particle size of 2.7 μm. The densely bonded, extensively endcapped diphenylmethyl bonded phase of BIOshell^™ IgG 1000 Å Diphenyl provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds, such as proteins.

Application

UHPLC Analysis of Reduced IgG1 on BIOshell^™ IgG 1000 Å Diphenyl
UHPLC Analysis of IgG2 on BIOshell^™ IgG 1000 Å C4, C18, and Diphenyl, 2.7 μm

Analysis Note

Target analytes include: monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights up to 500 kDa.

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable