Flow direction is marked on the column. Initial care should be taken to avoid mobile phases that are immiscible with acetonitrile or could cause a precipitate. Complete operation guidelines are found on the certificate of analysis.
577430-U
BIOshell™ IgG 1000 Å Diphenyl (2.7 μm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
Synonym(s):
BIOshell™ IgG 1000 Å Diphenyl, 2.7 μm HPLC Column
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$1,230.00
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About This Item
UNSPSC Code:
41115700
NACRES:
SB.52
Particle size:
2.7 μm
Matrix active group:
bonding phase, Diphenyl (diphenylmethyl)
Pore size:
1000 Å pore size
Matrix:
Fused-Core® particle platform, superficially porous particle
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Product Name
BIOshell™ IgG 1000 Å Diphenyl, 2.7 μm HPLC Column, column L × I.D. 15 cm × 4.6 mm
material
stainless steel column
agency
suitable for USP L11
feature
endcapped
packaging
1 ea of
extent of labeling
1.0% loading
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
column L × I.D.
15 cm × 4.6 mm
surface area
22 m2/g
matrix
Fused-Core® particle platform, superficially porous particle
matrix active group
bonding phase, Diphenyl (diphenylmethyl)
particle size
2.7 μm
pore size
1000 Å pore size
operating pH range
2-9
separation technique
reversed phase
product line
BIOshell™
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This Item | 577428-U | 577427-U | 577420-U |
|---|---|---|---|
| particle size 2.7 μm | particle size 2.7 μm | particle size 2.7 μm | particle size 2.7 μm |
| separation technique reversed phase | separation technique reversed phase | separation technique reversed phase | separation technique reversed phase |
| matrix active group bonding phase, Diphenyl (diphenylmethyl) | matrix active group bonding phase, Diphenyl (diphenylmethyl) | matrix active group bonding phase, Diphenyl (diphenylmethyl) | matrix active group bonding phase, Diphenyl (diphenylmethyl) |
| pore size 1000 Å pore size | pore size 1000 Å pore size | pore size 1000 Å pore size | pore size 1000 Å pore size |
| matrix Fused-Core® particle platform, superficially porous particle | matrix Fused-Core® particle platform, superficially porous particle | matrix Fused-Core® particle platform, superficially porous particle | matrix Fused-Core® particle platform, superficially porous particle |
| technique(s) HPLC: suitable, UHPLC-MS: suitable, LC/MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable |
Analysis Note
Target analytes include: monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights up to 500 kDa.
Legal Information
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
BIOshell is a trademark of Sigma-Aldrich Co. LLC
Application
General description
BIOshell™ IgG 1000 Å Diphenyl is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000 Å) Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thin porous shell and the small overall particle size of 2.7 μm. The densely bonded, extensively endcapped diphenylmethyl bonded phase of BIOshell™ IgG 1000 Å Diphenyl provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds, such as proteins.
Storage Class
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Bonjour, j'aurais aimé savoir quelles sont les spécifications à suivre pour la mise en service de cette colonne BIOshell Diphényl. La colonne étant stockée en 100% ACN, quelles sont les recommandations à suivre pour la mettre en service ? Merci !
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