577438-U

BIOshell^™ IgG 1000 Å Diphenyl (2.7 μm) HPLC Columns

L × I.D. 5 cm × 500 μm, HPLC Column

• Synonym(s): BIOshell^™ IgG 1000 Å Diphenyl, 2.7 μm HPLC Column
• UNSPSC Code: 41115709
• NACRES: SB.52
• Particle size: 2.7 μm
• Matrix active group: bonding phase, Diphenyl (diphenylmethyl)
• Pore size: 1000 Å pore size
• Matrix: Fused-Core^® particle platform

Properties

• Product Name: BIOshell^™ IgG 1000 Å Diphenyl, 2.7 μm HPLC Column, column L × I.D. 5 cm × 500 μm
• material: stainless steel hardware
• agency: suitable for USP L11
• feature: endcapped
• packaging: pkg of 1 unit
• extent of labeling: 1.0% loading
• technique(s): HPLC: suitable
• column L × I.D.: 5 cm × 500 μm
• surface area: 22 m²/g
• matrix: Fused-Core^® particle platform
• matrix active group: bonding phase, Diphenyl (diphenylmethyl)
• particle size: 2.7 μm
• pore size: 1000 Å pore size
• operating pH range: 2-9
• separation technique: reversed phase
• Quality Level: 100
• product line: BIOshell^™

Description

Analysis Note

Target analytes include: monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights up to 500 kDa.

Legal Information

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Application

UHPLC Analysis of Reduced IgG1 on BIOshell^™ IgG 1000 Å Diphenyl
UHPLC Analysis of IgG2 on BIOshell^™ IgG 1000 Å C4, C18, and Diphenyl, 2.7 μm

General description

BIOshell^™ IgG 1000 Å Diphenyl is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000 Å) Fused-Core^® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thin porous shell and the small overall particle size of 2.7 μm. The densely bonded, extensively endcapped diphenylmethyl bonded phase of BIOshell^™ IgG 1000 Å Diphenyl provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds, such as proteins.