581508-U

Ascentis^® Si (5 µm) HPLC Columns

Name: Ascentis<SUP>&#174;</SUP> Si (5 µm) HPLC Columns
Brand: SUPELCO
Price: 808 USD

L × I.D. 10 cm × 2.1 mm, HPLC Column

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1 EA

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About This Item

UNSPSC Code:

41115700

eCl@ss:

32110501

NACRES:

SB.52

L × i.d.:

10 cm × 2.1 mm

Particle size:

5 μm

Matrix active group:

silica phase

Pore size:

100 Å

Matrix:

fully porous particle, silica gel high purity, spherical

Key Documents

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Product Name

Ascentis^® Si HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm

material

stainless steel column

agency

suitable for USP L3

product line

Ascentis^®

feature

endcapped: no

manufacturer/tradename

Ascentis^®

packaging

1 ea of

parameter

≤70 °C temp. range, 400 bar pressure (5801 psi)

technique(s)

HPLC: suitable, LC/MS: suitable

L × I.D.

10 cm × 2.1 mm

surface area

450 m²/g

impurities

<5 ppm metals

matrix

fully porous particle, silica gel high purity, spherical

matrix active group

silica phase

particle size

5 μm

pore size

100 Å

operating pH range

2-6

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC), normal phase

Quality Level

100

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This Item	581514-U	581513-U	581502-U
Supelco 581508-U Ascentis^® Si HPLC Column	Supelco 581514-U Ascentis^® Si HPLC Column	Supelco 581513-U Ascentis^® Si HPLC Column	Supelco 581502-U Ascentis^® Si HPLC Column
matrix active group silica phase	matrix active group silica phase	matrix active group silica phase	matrix active group silica phase
separation technique hydrophilic interaction (HILIC), normal phase	separation technique hydrophilic interaction (HILIC), normal phase	separation technique hydrophilic interaction (HILIC), normal phase	separation technique hydrophilic interaction (HILIC), normal phase
particle size 5 μm	particle size 5 μm	particle size 5 μm	particle size 3 μm
L × I.D. 10 cm × 2.1 mm	L × I.D. 25 cm × 10 mm	L × I.D. 25 cm × 4.6 mm	L × I.D. 15 cm × 2.1 mm
pore size 100 Å	pore size 100 Å	pore size 100 Å	pore size 100 Å
agency suitable for USP L3	agency suitable for USP L3	agency suitable for USP L3	agency suitable for USP L3

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis Si is a high loading capacity silica with excellent peak shape. The Ascentis Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Simultaneous determination of ozone and carbonyls using trans-1,2-bis(4-pyridyl)ethylene as an ozone scrubber for 2,4-dinitrophenylhydrazine-impregnated silica cartridge.

Shigehisa Uchiyama et al.

Analytical chemistry, 80(9), 3285-3290 (2008-03-18)

A new method for the simultaneous determination of ozone and carbonyls in air using a two-bed cartridge system has been developed. Each bed consists of reagent-impregnated silica particles. The first contains trans-1,2-bis-(4-pyridyl) ethylene (BPE) while the second contains 2,4-dinitrophenylhydrazine (DNPH).

"Dilute & shoot" approach for rapid determination of trace amounts of nicotine in zero-level e-liquids by reversed phase liquid chromatography and hydrophilic interactions liquid chromatography coupled with tandem mass spectrometry-electrospray ionization.

Paweł Kubica et al.

Journal of chromatography. A, 1289, 13-18 (2013-04-04)

Two analytical procedures are proposed where HILIC and RPLC techniques are coupled with tandem mass spectrometry detection for rapid determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes. Samples are prepared on the basis of the approach

Characterization of Ascentis RP-Amide column: Lipophilicity measurement and linear solvation energy relationships.

Deborah Benhaim et al.

Journal of chromatography. A, 1217(1), 65-74 (2009-11-27)

This study investigates lipophilicity determination by chromatographic measurements using the polar embedded Ascentis RP-Amide stationary phase. As a new generation of amide-functionalized silica stationary phase, the Ascentis RP-Amide column is evaluated as a possible substitution to the n-octanol/water partitioning system

Characterization of new types of stationary phases for fast liquid chromatographic applications.

Szabolcs Fekete et al.

Journal of pharmaceutical and biomedical analysis, 50(5), 703-709 (2009-06-30)

The performance of a narrow bore silica based monolith column (5 cm x 2 mm) was compared to 5 cm long narrow bore (internal diameter < or = 2.1 mm) columns, packed with shell particles (2.7 microm) and totally porous

Shell and small particles; evaluation of new column technology.

Szabolcs Fekete et al.

Journal of pharmaceutical and biomedical analysis, 49(1), 64-71 (2008-11-29)

The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 microm particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities

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Protocols

Deriviatization, Hydrolysis, and HPLC Analysis of Amino Acids on SUPELCOSIL™ and ASCENTIS® Columns

Amino Acid Analysis is the suitable tool for precise determination of protein quantities but also provides detailed information regarding the relative amino.

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Ascentis® Si (5 µm) HPLC Columns