58298

Supelco

SUPELCOSIL LC-18 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

eCl@ss:
32110501

Quality Level

Agency/Method

suitable for USP L1

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

11.0% carbon loading

parameter

≤70 °C temp. limit
400 bar pressure (5801 psi)

application(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface coverage

surface coverage 3.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

surface area

170 m2/g

pH range

2 - 7.5

Featured Industry

Food and Beverages

separation technique

reversed phase

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General description

A general purpose hydrophobic alkyl phase that is very retentive and gives good peak shape for a wide variety of compounds.

Application

SUPELCOSIL LC-18 HPLC Column may be used for the separation of:
  • Anthocyanins, flavonols, and carbohydrates in Tropea red onions (Allium cepa L.) during their determination by high performance liquid chromatography (HPLC) as well as HPLC coupled to mass spectrometry (MS) equipped with electrospray ionization (ESI) source.
  • Sinapyl derivatives in pineapple (Ananas comosus [L.] Merill) juice samples during their determination by semipreparative reversed phase HPLC.
  • Aflatoxin M1 in milk and cheese samples during its determination by HPLC with post-column derivatization and fluorescence detection.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
High-performance liquid chromatography with post-column derivatisation and fluorescence detection for sensitive determination of aflatoxin M1 in milk and cheese.
Manetta AC, et al.
Journal of Chromatography A, 1083(1-2), 219-222 (2005)
Flavonoid and carbohydrate contents in Tropea red onions: effects of homelike peeling and storage.
Gennaro L, et al.
Journal of Agricultural and Food Chemistry, 50(7), 1904-1910 (2002)
Characterization of sinapyl derivatives in pineapple (Ananas comosus [L.] Merill) juice.
Wen L, et al.
Journal of Agricultural and Food Chemistry, 47(3), 850-853 (1999)
Protocols
Separation of δ-Tocopherol, analytical standard; Retinyl acetate; (+)-γ-Tocopherol; α-Tocopherol, ≥95.5%; Retinol; Vitamin A (acetate), meets USP testing specifications.
Read More
Separation of Methyl decanoate; Methyl dodecanoate; Methyl myristate; Methyl palmitate; Methyl caprylate; Methyl oleate; Methyl linoleate; Methyl linolenate; Methyl stearate
Read More

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