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58365

SUPELCOSIL™ LC-Si (5 µm) HPLC Columns

L × I.D. 25 cm × 10 mm, HPLC Column

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$2,390.00

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
NB.21
L × i.d.:
25 cm × 10 mm
Particle size:
5 μm
Matrix active group:
silica phase
Pore size:
120 Å pore size
Matrix:
silica gel, spherical particle platform

$2,390.00

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Product Name

SUPELCOSIL™ LC-Si HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm

agency

suitable for USP L3

Quality Level

100

manufacturer/tradename

SUPELCOSIL™

parameter

≤70 °C temp. limit, 400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 10 mm

surface area

170 m2/g

matrix

silica gel, spherical particle platform

matrix active group

silica phase

particle size

5 μm

pore size

120 Å pore size

pH range

2-7.5

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC), normal phase

General description

A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent. Non-polar compounds will elute from the column first, while polar solutes show stronger interaction with the silanol groups on the silica surface. The polar selectivity of silica is very helpful for separating mycotoxins. Silica is frequently used in preparative chromatography due to its low operating cost and back pressure compared to reversed-phase columns. Silica is particularly popular among organic chemists as a low-pressure preparative tool. The unmodified silica in SUPELCOSIL LC-Si columns also separate positional isomers.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Supelco

59166

SUPELCOSIL™ LC-Si HPLC Column

particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

matrix active group

silica phase

matrix active group

silica phase

matrix active group

silica phase

matrix active group

silica phase

L × I.D.

25 cm × 10 mm

L × I.D.

5 cm × 4.6 mm

L × I.D.

25 cm × 4.6 mm

L × I.D.

30 cm × 4 mm

pore size

120 Å pore size

pore size

120 Å pore size

pore size

120 Å pore size

pore size

120 Å pore size

agency

suitable for USP L3

agency

suitable for USP L3

agency

suitable for USP L3

agency

suitable for USP L3

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Certificates of Analysis (COA)

Lot/Batch Number
262974-01
262961-01
255207-01
255174-01
177361-01

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R Lovlin et al.
Journal of chromatography. B, Biomedical applications, 679(1-2), 196-198 (1996-04-26)
The stereospecific HPLC assays reported for ketoprofen (KT) mainly utilize indirect approaches. These assays involve the formation of amide diastereomeric derivatives, which are then separated by chromatography. The advantages of indirect methods include versatility, good sensitivity and cost effectiveness; however
Mohammed Shahid Ali et al.
Journal of pharmaceutical and biomedical analysis, 43(1), 158-167 (2006-08-05)
A new HILIC method has been developed for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold syrup. Mobile phase consists of methanol:water (containing 6.0 g of ammonium acetate and 10 mL of
J J Agren et al.
Lipids, 37(6), 613-619 (2002-07-18)
Normal-phase HPLC resolution of sn-1,2(2,3)- and x-1,3-DAG generated by partial Grignard degradation from natural TAG was carried out with both (R)-(-) and (S)-(+)-1-(1-naphthyl)ethylurethane derivatives. The diastereomeric sn-1,2- and sn-2,3-DAG derivatives were resolved using two Supelcosil LC-Si (5 microm, 25 cm

Global Trade Item Number

SKUGTIN
5836504061833669242

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