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58367

Supelco

SUPELCOSIL LC-8 HPLC Column

5 μm particle size, L × I.D. 25 cm × 10 mm

eCl@ss:
32110501
Pricing and availability is not currently available.

Quality Level

Agency/Method

suitable for USP L7

feature

endcapped

mfr. no.

SUPELCOSIL

extent of labeling

6.0% carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

application(s)

HPLC: suitable

L × I.D.

25 cm × 10 mm

surface coverage

surface coverage 3.2 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

C8 (octyl) phase

particle size

5 μm

pore size

120 Å

surface area

170 m2/g

pH range

2 - 7.5

Featured Industry

Food and Beverages

separation technique

reversed phase

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General description

A phase less hydrophobic than C18. Provides less retention of both polar and non-polar compounds than C18. Use a mobile phase containing 5% less organic modifier for the C8 column than C18. Polar compounds are, relatively, more strongly retained on C8 than C18 columns.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
Certificate of Origin
F Belal et al.
Journal of pharmaceutical and biomedical analysis, 24(3), 335-342 (2001-02-24)
A simple, rapid and sensitive HPLC method has been developed for the simultaneous determination of ramipril and hydrochlorothiazide in their dosage forms. Acetonitrile: sodium perchlorate solution (0.1 M) adjusted to pH 2.5+/-0.2 with phosphoric acid (46:54 v/v), was used as...
Thomas Borch et al.
Journal of chromatography. A, 1022(1-2), 83-94 (2004-02-03)
Explosives such as 2,4,6-trinitrotoluene (TNT), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), and hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) are widely distributed environmental contaminants. Complete chromatographic separation is necessary in order to accurately determine and quantify explosives and their degradation products in environmental samples and in (bio)transformation studies. The...
Jadwiga Piwowarska et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation...
K Ary et al.
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium...
P K Kunicki
Journal of chromatography. B, Biomedical sciences and applications, 750(1), 41-49 (2001-02-24)
A HPLC-UV determination of clobazam and N-desmethylclobazam in human serum and urine is presented. After simple liquid-liquid extraction with dichloromethane the compounds and an internal standard diazepam were separated on a Supelcosil LC-8-DB column at ambient temperature under isocratic conditions...

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