584602-U

Ascentis^® Express 90 Å Biphenyl, 2.0 μm HPLC Column

column L × I.D. 15 cm × 2.1 mm

• Synonym(s): Ascentis Express 90 Å Biphenyl, 2.0 μm HPLC Column

Properties

• Agency/Method: suitable for USP L11
• feature: endcapped
• extent of labeling: 7.0% Carbon loading
• application(s): HPLC: suitable
• column L × I.D.: 15 cm × 2.1 mm
• matrix: Fused-Core^® particle platform
• matrix active group: Biphenyl (dimethylbiphenyl) bonding phase
• particle size: 2.0 μm
• pore size: 90 Å pore size
• surface area: 120 m²/g
• operating pH range: 2 - 9

Description

General description

Ascentis^® Express 90 Å Biphenyl is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core^® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 μm thick porous shell and the small overall particle size of 2 μm. The bonded, densely endcapped, dimethyl-biphenyl stationary phase provides a stable, reversed phase packing with enhanced pi-pi and mild steric interactions due to the two sequential phenyl groups bonded to the base silica. The 90 Å Biphenyl can be used for basic, acidic, or neutral compounds with alternate selectivity from C18 and is especially useful for separating peaks that elute early on C18 and other phenyl phases.

Application

UHPLC/MS Analysis of Pain Management Opiates
UHPLC/MS Analysis of SAMHSA 5 Panel on Ascentis^® Express Biphenyl, 2 μm

Analysis Note

Target analytes include electron-poor molecules, aromatic or unsaturated compounds (ketones, nitriles, alkenes).

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.