Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

59132

Supelco

SUPELCOSIL LC-NH₂ NP (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

SUPELCOSIL LC-NH2-NP 5UM 25CMX4.6MM HPLC COLUMN

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

SUPELCOSIL LC-NH2-NP HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

agency

suitable for USP L8

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

3% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 5.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

amino phase

particle size

5 μm

pore size

120 Å

pH

2-7.5

application(s)

food and beverages

separation technique

normal phase

General description

An amino phase dedicated to normal-phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal-phase chromatography. Normal-phase chromatography is especially useful when the analytes are not water soluble -- for example, the fat-soluble vitamins A, D, E, and K.

These columns should be used with non-aqueous mobile phases only.
SUPELCOSIL LC-NH2-NP is an amino phase dedicated to normal phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal phase chromatography.

Features and Benefits

• show stable retention in normal-phase separations
• are less sensitive to small or varying amounts of water in mobile phases, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
SUPELCOSIL LC-NH2-NP columns:
• show stable retention in normal phase separations
• are less sensitive to small or varying amounts of water in the mobile phase, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

S Djabarouti et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 799(1), 165-172 (2003-12-09)
The aim of this study was to develop a specific and sensitive high-performance liquid chromatographic (HPLC) assay for the determination of levofloxacin in human plasma, bronchoalveolar lavage and bone tissues. The sample extraction was based on a fully automated liquid-solid
Vijay V Upreti et al.
Biomedical chromatography : BMC, 17(6), 385-390 (2003-09-19)
A simple and sensitive high-performance liquid chromatography (HPLC) method has been developed and validated for the determination of DRF-1042, a novel orally active camptothecin (CPT) analog, in human plasma. The sample preparation was a simple deproteinization with acidified methanol yielding
E M Koves
Journal of chromatography. A, 692(1-2), 103-119 (1995-02-10)
A comprehensive approach to the analysis for many drugs in postmortem blood and biological fluids using high-performance liquid chromatography and diode array detection has been developed. To reduce the likelihood of co-eluting interference components of postmortem blood or other drugs
K K Peh et al.
International journal of pharmaceutical compounding, 4(3), 229-231 (2000-05-01)
A simple and selective high-performance liquid chromatography (HPLC) method using ultraviolet detection was developed for simultaneous determination of fusidic acid and betamethasone dipropionate in a cream formulation. A Supelcosil LC18 column was used for chromatographic separation. The mobile phase consisted
Kornélia Tekes
Journal of chromatographic science, 46(2), 169-173 (2008-03-28)
A sensitive, simple, and reliable high-performance liquid chromatographic method with electrochemical detection is developed for the measurement of four natural products, the serotonin-related indols from human platelet-rich plasma (PRP) using N-methylserotonin as internal standard. Separation of serotonin (5HT), 5-hydroxytryptophan (5HTP)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service