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59132

SUPELCOSIL™ LC-NH₂ NP (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

SUPELCOSIL™ LC-NH2-NP 5UM 25CMX4.6MM HPLC COLUMN

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
NB.21
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
amino phase
Pore size:
120 Å
Matrix:
silica gel, spherical particle platform

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Product Name

SUPELCOSIL™ LC-NH2-NP HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

agency

suitable for USP L8

feature

endcapped

manufacturer/tradename

SUPELCOSIL™

extent of labeling

3% Carbon loading

parameter

≤70 °C temp. range, 400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 5.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

amino phase

particle size

5 μm

pore size

120 Å

pH

2-7.5

application(s)

food and beverages

separation technique

normal phase

Quality Level

100

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This Item
58989C3058338C4058338C30
Supelco

Supelco

59132

SUPELCOSIL™ LC-NH2-NP HPLC Column

SUPELCOSIL™ LC-NH2 HPLC Column 3 μm particle size, L × I.D. 15 cm × 3 mm

Supelco

58989C30

SUPELCOSIL™ LC-NH2 HPLC Column

SUPELCOSIL™ LC-NH2 HPLC Column 5 μm particle size, L × I.D. 25 cm × 4 mm

Supelco

58338C40

SUPELCOSIL™ LC-NH2 HPLC Column

SUPELCOSIL™ LC-NH2 HPLC Column 5 μm particle size, L × I.D. 25 cm × 3 mm

Supelco

58338C30

SUPELCOSIL™ LC-NH2 HPLC Column

matrix active group

amino phase

matrix active group

amino phase

matrix active group

amino phase

matrix active group

amino phase

separation technique

normal phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

particle size

5 μm

particle size

3 μm

particle size

5 μm

particle size

5 μm

L × I.D.

25 cm × 4.6 mm

L × I.D.

15 cm × 3 mm

L × I.D.

25 cm × 4 mm

L × I.D.

25 cm × 3 mm

agency

suitable for USP L8

agency

suitable for USP L8

agency

suitable for USP L8

agency

suitable for USP L8

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

General description

An amino phase dedicated to normal-phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal-phase chromatography. Normal-phase chromatography is especially useful when the analytes are not water soluble -- for example, the fat-soluble vitamins A, D, E, and K.

These columns should be used with non-aqueous mobile phases only.
SUPELCOSIL LC-NH2-NP is an amino phase dedicated to normal phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal phase chromatography.

Features and Benefits

• show stable retention in normal-phase separations
• are less sensitive to small or varying amounts of water in mobile phases, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
SUPELCOSIL LC-NH2-NP columns:
• show stable retention in normal phase separations
• are less sensitive to small or varying amounts of water in the mobile phase, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Certificates of Analysis (COA)

Lot/Batch Number
299013-01
298454-01
298341-01
298340-01
298339-01

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K K Peh et al.
International journal of pharmaceutical compounding, 4(3), 229-231 (2000-05-01)
A simple and selective high-performance liquid chromatography (HPLC) method using ultraviolet detection was developed for simultaneous determination of fusidic acid and betamethasone dipropionate in a cream formulation. A Supelcosil LC18 column was used for chromatographic separation. The mobile phase consisted
F N Hussain et al.
Journal of chromatography. B, Biomedical sciences and applications, 716(1-2), 257-266 (1998-11-21)
We describe a sensitive high-performance liquid chromatographic method for the determination of 5-aminosalicylic acid and N-acetyl-5-aminosalicylic acid in rectal tissue biopsies. Samples were derivatised using propionic anhydride and proteins were precipitated with methanol. A Supelcosil ABZ column (150x4.6 mm I.D.
O Cudina et al.
Farmaco (Societa chimica italiana : 1989), 55(2), 125-127 (2000-04-27)
Fluocortolone and its esters are synthetic corticosteroids used topically in the treatment of various skin disorders. A method that can be successfully used for the separation and determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories was developed. This method
Thadikamala Sathish et al.
Pakistan journal of pharmaceutical sciences, 22(1), 68-73 (2009-01-27)
An accurate, simple, reproducible and sensitive RP-HPLC method for the determination of bharangin has been developed and validated. The separation of bharangin and 2-nitroaniline (internal standard) was achieved on Supelcosil LC-18 (3micro, 150 x 4.6 mm i.d.) column using UV
S Moret et al.
Zeitschrift fur Lebensmittel-Untersuchung und -Forschung, 201(4), 322-326 (1995-10-01)
This paper proposes a simple HPLC method for the determination of polycyclic aromatic hydrocarbons (PAHs) in water, wine and beer. Samples were purified by PAH collection in solid-phase extraction (SPE) and analysed by reversed-phase HPLC (Supelcosil LC-PAH column from Supelco).
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