5 μm particle size, L × I.D. 25 cm × 4.6 mm


Quality Level


suitable for USP L14



extent of labeling

12% carbon loading


0-70 °C temperature
400 bar pressure (5801 psi)


HPLC: suitable

L × I.D.

25 cm × 4.6 mm


silica gel, spherical particle platform

matrix active group

propyltrimethylammonium phase

particle size

5 μm

pore size

120 Å

surface area

170 m2/g

Featured Industry

Food and Beverages

separation technique

anion exchange

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General description

SUPELCOSIL SAX1 HPLC Column is typically employed as an anion exchange column in chromatographic separations.
The SUPELCOSIL SAX1 column has a strongly basic quaternary aminopropyl phase and is used for separating anions.


SUPELCOSIL SAX1 HPLC Column has been used for the separation of:
  • Organoarsenic compounds in marine algae and in biological materials during their analysis using size-exclusion-anion-exchange (SE-AE) high performance liquid chromatography (HPLC) combined with inductively coupled plasma mass spectrometry (ICP-MS).
  • Organoarsenic compounds in edible algae during their analysis using bi-dimensional SE-AE-HPLC combined with dual ICP-MS and electrospray tandem mass spectrometry (ESI-MS/MS).
  • Polypeptides in biological matrices during their analysis using supercritical fluid chromatography (SFC) coupled to MS detector.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
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Feasibility of supercritical fluid chromatography/mass spectrometry of polypeptides with up to 40-mers.
Zheng J, et al.
Analytical Chemistry, 78(5), 1535-1545 (2006)
Speciation of arsenic in edible algae by bi-dimensional size-exclusion anion exchange HPLC with dual ICP-MS and electrospray MS/MS detection.
McSheehy S and Szpunar J
Journal of Analytical Atomic Spectrometry, 15(1), 79-87 (2000)
Gas and liquid chromatography with inductively coupled plasma mass spectrometry detection for environmental speciation analysis-advances and limitations.
Szpunar J, et al.
Spectrochimica Acta. Part B: Atomic Spectroscopy, 55(7), 779-793 (2000)
Trace element speciation analysis of biomaterials by high-performance liquid chromatography with inductively coupled plasma mass spectrometric detection.
Szpunar J.
TrAC, Trends in Analytical Chemistry, 19(2-3), 127-137 (2000)
EPA Method 547 outlines the analysis of glyphosate in drinking water by direct aqueous injection HPLC, post column derivatization, and fluorescence detection
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