59183

Supelco

SUPELCOSIL LC-Si HPLC Column

12 μm particle size, L × I.D. 25 cm × 21.2 mm

eCl@ss:
32110501
Pricing and availability is not currently available.

Agency/Method

suitable for USP L3

mfr. no.

SUPELCOSIL

parameter

≤70 °C temp. limit
400 bar pressure (5801 psi)

application(s)

HPLC: suitable

L × I.D.

25 cm × 21.2 mm

matrix

silica gel, spherical particle platform

matrix active group

silica phase

particle size

12 μm

pore size

120 Å pore size

surface area

170 m2/g

pH range

2 - 7.5

Featured Industry

Food and Beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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General description

A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent. Non-polar compounds will elute from the column first, while polar solutes show stronger interaction with the silanol groups on the silica surface. The polar selectivity of silica is very helpful for separating mycotoxins. Silica is frequently used in preparative chromatography due to its low operating cost and back pressure compared to reversed-phase columns. Silica is particularly popular among organic chemists as a low-pressure preparative tool. The unmodified silica in SUPELCOSIL LC-Si columns also separate positional isomers.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
Certificate of Origin
J J Agren et al.
Lipids, 37(6), 613-619 (2002-07-18)
Normal-phase HPLC resolution of sn-1,2(2,3)- and x-1,3-DAG generated by partial Grignard degradation from natural TAG was carried out with both (R)-(-) and (S)-(+)-1-(1-naphthyl)ethylurethane derivatives. The diastereomeric sn-1,2- and sn-2,3-DAG derivatives were resolved using two Supelcosil LC-Si (5 microm, 25 cm...
Mohammed Shahid Ali et al.
Journal of pharmaceutical and biomedical analysis, 43(1), 158-167 (2006-08-05)
A new HILIC method has been developed for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold syrup. Mobile phase consists of methanol:water (containing 6.0 g of ammonium acetate and 10 mL of...
R Lovlin et al.
Journal of chromatography. B, Biomedical applications, 679(1-2), 196-198 (1996-04-26)
The stereospecific HPLC assays reported for ketoprofen (KT) mainly utilize indirect approaches. These assays involve the formation of amide diastereomeric derivatives, which are then separated by chromatography. The advantages of indirect methods include versatility, good sensitivity and cost effectiveness; however...

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