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64065-U

Supelco

Ascentis® Express Biphenyl Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

NACRES:
SB.52
Pricing and availability is not currently available.

material

stainless steel hardware

Quality Level

feature

endcapped

mfr. no.

Ascentis

parameter

60 °C max. temp.
9000 psi max. pressure (600 bar)

application(s)

HPLC: suitable
UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

impurities

<5 ppm metals

matrix

particle platform (Fused-Core)

matrix active group

biphenyl phase

particle size

2.7 μm

pore size

90 Å pore size

operating pH

1.5 - 9

suitability

suitable for L11 per USP

Featured Industry

Food and Beverages

separation technique

reversed phase

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General description

The Ascentis® Express biphenyl phase chemistry offers alternative selectivity for pharmaceutical analytes and drug metabolites that are not well retained or difficult to resolve on traditional alkyl (C18) and phenyl bonded phases. Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 ?m particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UHPLC users, as well as conventional HPLC users. Visit the Ascentis Express home page for more information on this new column technology.

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
Articles
An online SPE-LC/MS method has been developed for the rapid detection of thyroid hormones in human serum with minimal hands on effort and time-consuming steps.
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Tetrahydrocannabinolic acid A solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material.
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The bonded, densely endcapped, dimethyl-biphenyl stationary phase of Ascentis® Express 90 Å Biphenyl, based on fused-core particles, provides a stable, reversed phase packing with enhanced π-π and mild steric interactions due to the two sequential phenyl groups bonded to the base silica.
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Protocols
Potency testing in marijuana-infused edibles is an important problem that analytical labs are facing due to the complexity of the involved matrices. Concentration of active ingredients in these edibles can range from a few parts per million to 3.5 parts per thousand. This application demonstrates the extraction and HPLC-UV analysis of the active compounds.
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Thyroid hormones play critical roles in the regulation of biological processes, such as growth, metabolism, protein synthesis, and brain development. For a clinical laboratory, fast and reliable measurements of total hormone levels are important for the diagnosis and monitoring of thyroid diseases.
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Rapid potency testing of marijuana-infused edibles using LC/MS on a biphenyl stationary phase detected eleven cannabinoids.
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