TSKgel® Amide-80 HPLC Column

phase carbamoyl, L × I.D. 30 cm × 21.5 mm, 10 μm particle size

TSKgel Amide-80, 30 cm x 21.5 mm ID, 10 μm
Pricing and availability is not currently available.


stainless steel hardware

mfr. no.

Tosoh, 14460


0-100% organic solvent
10-80 °C temperature
30 bar max. pressure


HPLC: suitable

L × I.D.

30 cm × 21.5 mm


silica particle platform

matrix active group

carbamoyl phase

particle size

10 μm

pore size

100 Å

surface area

450 m2/g

operating pH

2.0 - 7.5


suitable for L68 per USP

separation technique

hydrophilic interaction (HILIC)

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General description

TSKgel Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.

Physical form

Shipped in 85% acetonitrile - 15% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation
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