816257

TSKgel^® Octadecyl-4PW (13 µm) HPLC Columns

L x I.D. 15 cm × 21.5 mm, C18 (octadecyl) phase

• Synonym(s): TSKgel^® Reversed Phase HPLC Column (Polymer-based)
• UNSPSC Code: 41115711
• eCl@ss: 32110501

Properties

• product name: TSKgel^® Octadecyl-4PW HPLC Column, phase C18 (octadecyl), L × I.D. 15 cm × 21.5 mm, 13 μm particle size
• material: stainless steel hardware
• product line: TSKgel^®
• manufacturer/tradename: Tosoh 16257
• extent of labeling: 1 meq/mL ligand density (estimate)
• parameter: no limitation salt concentration; 0-100% organic solvent; 30 bar max. pressure; 5-50 °C temperature; 8 mL/min flow rate
• technique(s): HPLC: suitable
• L × I.D.: 15 cm × 21.5 mm
• matrix: polymer particle platform
• matrix active group: C18 (octadecyl) phase
• particle size: 13 μm
• pore size: 500 Å
• operating pH: 2-12
• separation technique: reversed phase

Description

General description

Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-4PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 500Å pore allows for anaylsis of proteins up to 200kDa while the particle size offerings allow for analytical, semi-preparative and preparative scale separations.

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.

• Octadecyl-2PW particles have 125Å pores
• Octadecyl-4PW particles have 500Å pores
• Phenyl-5PW RP particles have 1000Å pores
• Octadecyl-NPR particles are nonporous

Physical form

Shipped in 60% methanol - 40% waters (v/v).

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation