TSKgel® Alpha-5000 HPLC Column

phase hydroxylated methacrylate, L × I.D. 30 cm × 7.8 mm, 10 μm particle size

TSKgel Alpha Columns


stainless steel hardware


Tosoh 18342


≤0.5 M salt concentration
0-100% polar organic solvent
1 mL/min flow rate
30 bar max. pressure
80 °C temperature


HPLC: suitable

L × I.D.

30 cm × 7.8 mm


polymer particle platform

matrix active group

hydroxylated methacrylate phase

particle size

10 μm

pore size

1000 Å

operating pH

2 - 12


suitable for L38 per USP
suitable for L39 per USP

separation technique

size exclusion (SEC)

General description

TSKgel Alpha-5000 columns are packed with rigid, porous, polymer beads which show minimal shrinkage or swelling in different solvent systems. They are best used for the separation of polymers that are soluble in polar organic solvents such as methanol, ethanol, DMF, THF, HFIP, DMSO, 2-propanol, and acetonitrile.
TSKgel Alpha columns are composed of spherical, hydrophilic methacrylate beads. Unlike TSKgel PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel Alpha-type columns are stable in a wide variety of organic solvents at concentrations up to 100%. The main application area for TSKgel Alpha columns is the analysis of polymers that are soluble in polar organic solvents. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in DMF with 10mmol/L DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in HFIP. The mixed-bed column, TSKgel Alpha-M, has an extended linear calibration range, and is suitable for samples with a broad MW distribution as well as samples with unknown molecular weight.

Physical form

Shipped in water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation
Certificate of Analysis
Certificate of Origin

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