819696

TSKgel® Amide-80 HPLC Column

phase carbamoyl, L × I.D. 15 cm × 2 mm, 5 μm particle size

Synonym(s):
TSKgel Amide-80, 15 cm x 2 mm ID, 5 μm
eCl@ss:
32110501
Pricing and availability is not currently available.

material

stainless steel hardware

mfr. no.

Tosoh, 19696

parameter

0-100% organic solvent
10-80 °C temperature
100 bar max. pressure

application(s)

HPLC: suitable

L × I.D.

15 cm × 2 mm

matrix

silica particle platform

matrix active group

carbamoyl phase

particle size

5 μm

pore size

100 Å

surface area

450 m2/g

operating pH

2.0 - 7.5

suitability

suitable for L68 per USP

separation technique

hydrophilic interaction (HILIC)

Looking for similar products? Visit Product Comparison Guide

General description

TSKgel Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

TSKgel is a registered trademark of Tosoh Corporation
Certificate of Analysis
Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.