TSKgel® Amide-80 HPLC Column

phase carbamoyl, L × I.D. 25 cm × 4.6 mm, 5 μm particle size

TSKgel Amide-80 HR
Pricing and availability is not currently available.


stainless steel hardware


Amide-80 HR

mfr. no.

Tosoh, 21982


0-100% organic solvent
10-80 °C temperature
150 bar max. pressure


HPLC: suitable

L × I.D.

25 cm × 4.6 mm


silica particle platform

matrix active group

carbamoyl phase

particle size

5 μm

pore size

100 Å

surface area

450 m2/g

operating pH

2.0 - 7.5


suitable for L68 per USP

separation technique

hydrophilic interaction (HILIC)

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General description

TSKgel Amide-80 columns are now also available packed with 2 μm spherical silica particles, offering higher resolution and faster analysis with equivalent retention and selectivity as TSKgel Amide-80, 3 μm columns. This allows for smooth method transfer from a 3 μm to a 2 μm column. In addition, TSKgel Amide-80, 2 μm columns more highly retain hydrophilic compounds than competitive amide columns on the market.
Packed with 2, 3, 5, or 10 μm spherical silica particles that are covalently bonded with carbamoyl groups, TSKgel Amide-80 columns are used for the analysis of saccharides, glycans, oligosaccharides, peptides and polar compounds from natural products. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography.
TSKgel Amide-80 HR, 5 μm columns offer higher specifications for plate count compared to traditional TSKgel Amide-80, 5 μm, 4.6 mm ID x 25 cm columns (N = > 18,000 vs. 8,000). This equates to more efficient chromatography.

Physical form

Shipped in 75% acetonitrile - 25% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation
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