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MilliporeSigma

G005630

HPLC Analysis of Tricyclic Antidepressants on Ascentis® ES Cyano

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis ES Cyano, 15 cm x 4.6 mm I.D., 5 μm particles (577306-U)
mobile phase: [A] 10 mM potassium phosphate dibasic, pH 7 with phosphoric acid; [B] acetonitrile; [C] methanol; (25:60:15, A:B:C)
flow rate: 2 mL/min
column temp.: 25 °C
detector: UV, 215 nm
injection: 5 μL
sample: 100 μg/mL in water: methanol (70:30)

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Analysis Note

This application demonstrates the suitability of Ascentis ES Cyano for the efficient separation of tricyclic antidepressants.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Doxepin
    Trimipramine maleate salt
    Amitriptyline hydrochloride
    Imipramine
    Nordoxepin
    Nortriptyline
    Desipramine
    Protriptyline hydrochloride
    See All (8)

related product

Product No.
Description
Pricing

577306-U

Ascentis® ES-Cyano HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

A-923

Amitriptyline hydrochloride solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

D-075

N-Desmethyldoxepin-D3 hydrochloride solution, cis/trans, 100 μg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

D-906

Desipramine hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

D-927

Doxepin hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

I-902

Imipramine hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

N-907

Nortriptyline hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

P-903

Protriptyline hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

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