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1148090

USP

Collagenase II

United States Pharmacopeia (USP) Reference Standard

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Synonym(s):
Collagenase from Clostridium histolyticum, Clostridiopeptidase A
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.24

grade

pharmaceutical primary standard

manufacturer/tradename

USP

application(s)

pharmaceutical

format

neat

storage temp.

−70°C

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This Item
1148089C0130C1889
Collagenase II United States Pharmacopeia (USP) Reference Standard

1148090

Collagenase II

Collagenase I United States Pharmacopeia (USP) Reference Standard

1148089

Collagenase I

grade

pharmaceutical primary standard

grade

pharmaceutical primary standard

grade

-

grade

-

format

neat

format

neat

format

-

format

-

application(s)

pharmaceutical

application(s)

pharmaceutical

application(s)

-

application(s)

diagnostic assay manufacturing

storage temp.

−70°C

storage temp.

-

storage temp.

2-8°C

storage temp.

−20°C

General description

Collagenase II is a metalloprotease that acts as an endoprotease and exhibits a tripeptidyl carboxypeptidase activity. It shows endopeptidic activity with the main cleavage site found in front of the human collagen duplex amino acids glycine-proline. Hydrolysis takes place in the interior of the triple helical domains of collagen. Collagenase II is also known as class II collagenase and consists of three subtypes: δ, ε, and ζ. Collagenase II USP reference standard is provided as delivered and specified by the issuing Pharmacopoeia. All information provided in support of this product, including MSDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia. For further information and support please go to the website of the issuing Pharmacopoeia.

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Analysis Note

These products are for test and assay use only. They are not meant for administration to humans or animals and cannot be used to diagnose, treat, or cure diseases of any kind.  ​

Other Notes

This product is part of the USP Biologics program.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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