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HPLC Columns

A collection of various Supelco<sup>®</sup> HPLC and UHPLC columns scattered on a green surface. These columns, which are cylindrical with metallic finishes and equipped with fittings at both ends, represent a range of chromatography column technologies for different analytical needs. They vary in size and some have labeling that indicates specifications or model numbers.

Supelco® HPLC and UHPLC columns meet today’s demands of fast U/HPLC, LC-MS, and biopolymer separation, as well as regulated pharmacopeia and agency methods within various industries. Supelco® HPLC products are available with Fused-Core® particle technology, monolithic silica, and fully porous particles, including ultra-pure silica. In addition, porous graphitic carbon (PGC) particles, and polymeric particles are available for high pH stability.

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Related Resources

  • Brochure: HPLC Column Selection Guide

    The comprehensive Supelco® portfolio of analytical solutions to meet your U/HPLC and LC-MS needs.

  • Brochure: HPLC Method Development Guide

    High performance liquid chromatography (HPLC) is a technique for separating analytes dissolved in a liquid, mobile phase by using their specific interaction with a stationary phase (chromatography column).

  • Brochure: Ascentis® Express Columns

    Based on Fused-Core® particle technology, Ascentis® Express columns provide a exceptional advancements in HPLC column performance and the benefits of high sample throughput at maximum resolution.

  • Brochure: BIOshell™ Columns

    The BIOshell™ line of superficially porous particle (SPP) packed columns has been developed to aid research into understanding the subtleties of the molecule that is being developed.

  • Brochure: Chromolith® HPLC Columns

    To truly accelerate chromatographic separations, there is no better choice than Chromolith® HPLC columns. Due to their revolutionary, monolithic technology, Chromolith® columns provide excellent and rapid separations with extremely high robustness and matrix tolerance compared to particulate columns.

  • Brochure: Purospher STAR Columns

    The success of any method depends on the quality of the stationary phase. Precise, long-term reproducibility is a key factor in achieving reliable results. The base silica of Purospher™ STAR columns is 99.999% pure.

  • Brochure: Chiral HPLC Columns

    Columns for versatile and robust Chiral HPLC and LC-MS separations.

  • Brochure: HPLC Accessories

    The use of HPLC systems to achieve faster analyses and increased resolution pushes systems toward higher operating pressures. For instance, faster flow rates, longer column lengths, and reduced particle sizes gain a performance advantage in HPLC, but at a cost of increased operating pressures.

  • Brochure: Discovery Bio Columns

    Many of the challenges facing researchers in the proteomics and biopharmaceutical fields are related to the need to obtain as much information as possible on very limited samples. We designed Discovery® BIO Wide Pore reversed-phase HPLC columns to address these challenges.

  • Brochure: LC-MS Resource Guide

    In this guide, we provide a premier selection of proven Supelco® tools and consumables that meet the requirements of scientists who primarily use the LC-MS technique for analysis of drugs, biomolecules separation and analytical assays.

  • Brochure: LC-MS Contaminants

    In this technical bulletin, you will learn some tips on identifying LC-MS contaminants and avoiding contamination. Our 110 years of separation expertise, combined with our precision-manufactured products, give you the greatest chance of obtaining reproducible, clean data.

  • Application Note: Supelco® HPLC Products: The Best Choice for Any LC Instrument

    Since the 1970’s, Supelco® has been a trusted name in high performance liquid chromatography (HPLC) consumables. From a diverse array of columns packed with different stationary phases to a complete portfolio of HPLC accessories, the Supelco® portfolio of HPLC consumables caters to the needs of all analysts.

  • Application Guide: Drug of Abuse

    Application compilation with focus on the LC-MS analysis of drugs of abuse in biological samples.

  • HPLC Method Transfer Calculator

    Easily transfer a method between HPLC columns, (scale between microbore through preparative range), based on the 2 column dimensions and current method conditions.

  • Wall Chart: HPLC Packings for USP Compendial Methods

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  • Application Guide: Ascentis® Express and BIOshell™ Column Cleaning Procedures and Best Practices

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Porous graphitic carbon (PGC) particles

Porous graphitic carbon (PGC) columns, like the Supel™ Carbon LC column, provide unique chromatographic properties that are unmatched by silica particle-packed columns.  The full pH range can be used as well as high temperature (> 200 °C), opening up new frontiers for chromatographers.

Superficially porous silica particles (SPP)/Fused Core® Technology

Superficially porous silica columns deliver higher speed and efficiency than fully porous particles of the same size. Fused-Core® HPLC columns like Ascentis® Express and BIOshell™ can convert any HPLC system into a fast HPLC workhorse with maximum speed and performance.

Monolithic silica

  • Monolithic, silica-based HPLC columns such as Chromolith® HPLC columns allow rapid separations at very low column backpressure with high matrix tolerance and extended column lifetime, thus making them ideal products for high throughput and cost-efficient analyses of matrix rich samples.
  • Chromolith® HPLC columns are made from monolithic silica with a bimodal pore structure having 2 µm macropores and 13 nm mesopores. This structure leads to various key advantages to the column.
  • Chromolith® WP columns are wide pore (300 Å) monolithic silica columns made up of a single continuous rod of high purity, porous silica. These columns have shown great potential compared to conventional columns with consistent performance over a longer lifetime. In addition to various RP phases, a Protein A affinity column and an epoxy column, that enable customer specific modifications, are  also available.

Fully porous silica particles (FPP) Type B (high purity silica)

  • Purospher® STAR HPLC and UHPLC columns are best suited for the peak-tailing free separation of basic, neutral, and metal chelating samples. Their superior performance and stability allow maximum flexibility in method development with extended pH stability.
  • Discovery® HPLC columns are efficient for the analysis of biomolecules and small molecules. These columns have polar and non-polar bondings, with unique retention, selectivity, and no-bleed properties for LC-MS applications.
  • The Ascentis® bonded phases have a wide range of selectivity. Type B silica makes the column inert, stable, and reproducible. The high surface area of the silica is suitable for  preparative loading capacity.
  • SeQuant® HILIC HPLC columns (hydrophilic interaction liquid chromatography) carry densely bonded, zwitterionic functional groups. These HILIC columns are available in a variety of lengths, particle sizes, and pore dimensions while showing an exceptional reproducibility and robustness.
  • Sepax® HPLC columns are highly porous silica-based columns mainly utilized for peptides and protein analysis.

Fully porous silica particles (FPP) Type A (conventional silica)

  • Supelcosil® silica-based HPLC column line has a wide range of bonded phase chemistries, with the particle sizes and column configurations ranging from microbore to preparative scale. These columns show great efficiency for polar, nonpolar, and charged analytes.
  • LiChrospher® and Superspher® HPLC columns are highly versatile and reliable, proven in thousands of established HPLC methods.
  • LiChrosorb® HPLC columns are packed with irregular silica particles ranging from analytical scale 5 µm to large particles sized for production scale.
  • TSKgel® HPLC and UHPLC columns are available for gel filtration, gel permeation, ion exchange, reversed-phase, hydrophobic interaction, hydrophilic interaction, and affinity chromatography. (only available in the US and Europe)

Fully porous polymeric particles

  • apHera™ reversed-phase and amino HPLC columns are stable at the pH range 2-12 and are amenable to washing with alkaline solutions. They are ideal for the analysis of peptides, small proteins, carbohydrates, polar drugs, and organic acids.
  • Supelcogel® HPLC columns are packed with polystyrene-divinylbenzene co-polymer (PS-DVB) making them chemically stable. The two variations of PS-DVB co-polymer (6% and 8% cross-linked) maximize the separation process of the samples.
  • Hamilton Polymeric Reversed Phase HPLC columns are extremely inert, efficient, and durable, with high mechanical and thermal stability.
  • TSKgel® HPLC and UHPLC columns are available for the separation of proteins, peptides, and other biomolecules. The columns containing silica and methacrylate particles with different pore sizes can accommodate analytes with a wide range of molecular weights. (only available in the US and Europe)

Chiral HPLC Columns

We have a wide range of chiral HPLC columns for various applications.





Related Webinars

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Webinar: Tips & Tricks to Improve MS Sensitivity

In this webcast, the origins and problems in LC–MS arising from impurities and contaminations will be described in detail and tips and tricks will be provided in order to enable or maintain high quality and high sensitivity LC-MS analyses.

An open laptop with a colorful display featuring a stylized human figure in blue on the right and a pink rectangle on the left against a yellow background.
Webinar: Supel™ Carbon LC Columns: New Tools for the Chromatographer’s Tool Box

Learn about porous graphitic carbon, a stationary phase material that is fully porous, carbon-based material—it is capable of analyzing small molecules and smaller proteins and peptides.

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Webinar: Improving Bioanalysis Workflows

Quantitation of monoclonal antibodies by LC-MS is a difficult analytical challenge due to the complexity in the workflow, from sample prep to LC-MS quantitation. This presentation will highlight a simple and high throughput method for the quantitation of monoclonal antibodies and proteins in human and animal serum.

An open laptop with a colorful display featuring a stylized human figure in blue on the right and a pink rectangle on the left against a yellow background.
Webinar: Reflecting on Chiral: Strategies and Tools for Efficient Enantiomer Separations

The focus of this seminar is to provide an overview of different strategies and methods employed over the past several years in characterizing samples containing chiral compounds.

An open laptop with a colorful display featuring a stylized human figure in blue on the right and a pink rectangle on the left against a yellow background.
Webinar: Advances in Liquid Chromatography Separations Employing Carbon Particles

This presentation focuses on recent advances, in the past two years, in 'carbon-HPLC' in the analysis of compounds classically associated with being difficult to separate by conventional 'silica-HPLC.'

An open laptop with a colorful display featuring a stylized human figure in blue on the right and a pink rectangle on the left against a yellow background.
Webinar: How to Increase The Cost Effectiveness for HPLC Separations of Challenging and Matrix-rich Samples

Join our webinar focusing on "How to Increase The Cost Effectiveness for HPLC Separations of Challenging and Matrix-rich Samples" for the potential for cost and time savings by increasing lab productivity with faster separations and column materials providing very high matrix tolerance.


*Tosoh Bioscience columns are available in the following countries:

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