Cloning & Expression
Investigating your gene of interest requires reliable reagents and resources for molecular cloning, transfection, and protein expression workflows. Our efficient restriction and DNA modifying enzymes and protein expression vector technologies are designed to ensure an effective cloning and protein expression strategy. In addition, our lipid and polymer-based transfection reagents are suitable for delivering DNA, siRNA, miRNA, and CRISPR/Cas components into the cell type best suited for your needs. Explore our expansive portfolio of cloning and protein expression reagents and select the tools you need to make your next breakthrough.
Transfection
Transfection reagents, protocols, and resources including Roche X-tremeGENE transfection reagents for DNA, siRNA, miRNA, and CRISPR/Cas9 gene editing components.
Molecular Cloning & Protein Expression
Molecular cloning and protein expression methods and protocols for reliable cloning and robust protein expression from insect, bacteria, and mammalian protein expression vectors.
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Dimethyl sulfoxide
Molecular Biology
Polybrene Infection / Transfection Reagent
A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.
DAPI
for nucleic acid staining
X-tremeGENE™ HP DNA Transfection Reagent
High-performance polymer reagent for transfecting many cell lines
LB Broth (Lennox)
Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.
LB Broth (Miller)
Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture
X-tremeGENE™ 9 DNA Transfection Reagent
Polymer reagent for transfecting common cell lines
Yeast Extract
for use in microbial growth medium
LB Broth (Miller)
Liquid microbial growth medium
Yeast Nitrogen Base Without Amino Acids
Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements
G-418 Solution
solution, =98% (TLC), suitable for transfection
LB Broth with agar (Miller)
Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.
GeneJuice® Transfection Reagent
Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.
LB Broth with agar (Lennox)
Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.
Sodium phosphate monobasic
BioReagent, Molecular Biology, anhydrous, ≥98%
LB Agar Plates
pre-poured LB agar plates
Hygromycin B
from Streptomyces hygroscopicus
Ribonuclease A from bovine pancreas
Molecular Biology, ≥70 Kunitz units/mg protein, lyophilized
2x YT medium
Powder microbial growth medium
X-tremeGENE™ 360 Transfection Reagent
Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines
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