Plasmid purification is commonly used for molecular cloning, transformation, and recombinant protein expression. Plasmid purification kits are available based on size of the bacterial culture and corresponding plasmid yield (miniprep, midiprep, maxiprep, megaprep, and gigaprep).
Our plasmid kits provide reliable isolation of high quality plasmid DNA. These kits are:
These products allow researchers to maximize the reproducibility of their workflow and minimize their time spent on obtaining plasmid DNA for transfection, sequencing, PCR, and other downstream applications.
GenElute™ plasmid miniprep kits offer simple, rapid, and cost-effective methods for isolating plasmid DNA from E. coli cultures. These kits combine silica-based membrane technology and the convenience of a spin column format.
GenElute™ HP plasmid purification kits yield high quality plasmid DNA in less than 30 minutes for Mini, Midi, and Maxiprep kits, or 1.5 hours for Mega and Gigaprep kits.
Roche Genopure™ midi and maxi prep kits use anion exchange chromatography for plasmid purification. A modified alkaline lysis protocol optimized for cell cultures grown in LB media is used to isolate DNA. The lysate can be filtered rather than centrifuged prior to anion exchange, resulting in shorter plasmid prep times and complete removal of SDS from the purified product.
Endotoxins reduce cell viability and transfection efficiencies among different eukaryotic cell lines and can influence the outcome and reproducibility of a given transfection experiment when using mammalian and other eukaryotic cells. GenElute™ endotoxin-free plasmid prep kits offer a simple, rapid, cost-effective method for purifying plasmid DNA with <0.1
EU/mg DNA for high-efficiency transfection.
GenElute™ five-minute miniprep kits feature a streamlined protocol yielding up to 5 mg high-quality plasmid DNA in about five minutes.
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