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RNA Purification

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RNA purification is a critical first step in many analyses due to the easy degradability of RNA. GenElute™ RNA purification kits enable purification of RNA from a variety of sources quickly and efficiently with minimal degradation, maximum purity, and high recovery and yield. GenElute™-E Single Spin RNA purification kits can be used to clean up RNA preps in a single spin for PCR and downstream applications.

Products

kits (18)

buffers (2)

enzymes (2)

RNA purification (12)

DNA extraction (2)

RT-PCR (2)

DNA purification (1)

LAMP (1)

Northern blotting (1)

Roche (4)
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TRI Reagent<SUP>®</SUP>
T9424

TRI Reagent®

For processing tissues, cells cultured in monolayer or cell pellets

RNA<I>later</I><SUP>®</SUP>
R0901

RNAlater®

Stabilize and protect RNA with immediate RNase inactivation

RNaseZAP<SUP>™</SUP>
R2020

RNaseZAP™

Cleaning agent for removing RNase

Spectrum<SUP>™</SUP> Plant Total RNA Kit
STRN50

Spectrum™ Plant Total RNA Kit

sufficient for 50 purifications

Spectrum<SUP>™</SUP> Plant Total RNA Kit
STRN250

Spectrum™ Plant Total RNA Kit

sufficient for 250 purifications

RNAzol<SUP>®</SUP> RT
R4533

RNAzol® RT

For processing total and small RNA from human, animal, plant, bacterial,and viral samples

High Pure RNA Tissue Kit
12033674001

High Pure RNA Tissue Kit

sufficient for 50 isolation(s), suitable for RT-PCR, suitable for Northern blotting

RNase AWAY<SUP>®</SUP>
83931

RNase AWAY®

decontamination reagent for RNase

GenElute<SUP>™</SUP> Mammalian Total RNA Miniprep Kit
RTN70

GenElute™ Mammalian Total RNA Miniprep Kit

sufficient for 70 purifications

High Pure miRNA Isolation Kit
05080576001

High Pure miRNA Isolation Kit

kit of for 50 isolations

mirPremier<SUP>®</SUP> microRNA Isolation Kit
SNC50

mirPremier® microRNA Isolation Kit

1 sufficient for 50 preparations

GenElute<SUP>™</SUP> Mammalian Total RNA Miniprep Kit
RTN350

GenElute™ Mammalian Total RNA Miniprep Kit

sufficient for 350 purifications

RNA/DNA Stabilization Reagent for Blood/Bone Marrow
11934317001

RNA/DNA Stabilization Reagent for Blood/Bone Marrow

GenElute<SUP>™</SUP> mRNA Miniprep Kit
MRN70

GenElute™ mRNA Miniprep Kit

sufficient for 70 purifications

GenElute<SUP>™</SUP> mRNA Miniprep Kit
MRN10

GenElute™ mRNA Miniprep Kit

sufficient for 10 purifications

Viral RNA Extraction Buffer
VRE100

Viral RNA Extraction Buffer

One Step RT-qPCR Compatible, Optimized for Viral RNA Detection in Saliva

GenElute<SUP>™</SUP> Mammalian Total RNA Miniprep Kit
RTN10

GenElute™ Mammalian Total RNA Miniprep Kit

sufficient for 10 purifications

GenElute<SUP>™</SUP>-E Single Spin RNA Cleanup Kit
EC800

GenElute™-E Single Spin RNA Cleanup Kit

Reagents and consumables for organic solvent clean-up from RNA. 10, 50 or 250 purifications.

High Pure RNA Paraffin Kit
03270289001

High Pure RNA Paraffin Kit

kit of for up to 100 isolations

Spectrum<SUP>™</SUP> Plant Total RNA Kit
STRN10

Spectrum™ Plant Total RNA Kit

sufficient for 10 purifications

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GenElute™ RNA Purification Kits

Classic GenElute™ RNA purification kits use silica or silicon carbide to strongly bind RNA, allowing proteins and other biological contaminants to be removed through wash steps. mRNA purification kits use oligo dT30 covalently linked to polystyrene beads to capture polyadenylated mRNA by hybridization. After washing, purified RNA is eluted. A broad portfolio of GenElute™ purification kits tailored to specific sample types are available, enabling purification of total RNA and mRNA from cells, tissues, serum, plasma, bacteria, viruses, and plant tissue. 

Features:

  • High yield, high purity RNA with minimal degradation
  • Comprehensive portfolio of kits optimized for purification of total RNA or mRNA from a variety of sample types
  • Offered in a variety of sizes and formats, from miniprep, midiprep, and maxiprep kits to high-throughput 96-well kits
  • Includes kits for RNA/DNA co-purification
  • Vacuum and spin formats

GenElute™-E single Spin RNA Purification Kits

GenElute™-E RNA purification kits use a negative chromatography technique dependent on size exclusion to isolate and purify nucleic acid in a single spin. Smaller molecules such as proteins become trapped in the pores of the stationary phase of the column. Larger RNA molecules flow through the column quickly because they are too large to enter the pores. By exploiting this difference in size, GenElute™-E purification kits enable RNA purification in a single spin without the need for multiple binding and wash steps.

Features:

  • Negative chromatography RNA purification method based on size exclusion
  • Simplified workflow
  • Quick protocol (3 minutes hands-on time, for a total of 15 minutes prep time)
  • Fewer chemical impurities, with no chaotropic salts or alcohol wash solutions that can inhibit downstream processes such as PCR
  • Reduced plastic waste and no hazardous binding or wash buffers
  • Kits tailored for RNA cleanup

RNA Purification Essentials

A variety of reagents are available to protect RNA from degradation. Stabilyser™ reagent is a proprietary formulation of detergents and solutes optimized for efficient extraction, stabilization, and storage of protein, DNA, and RNA from tissue samples. RNaseZAP™ reagent is a cleaning agent for removing RNase from glassware, plastic surfaces, countertops, and pipettors. RNAlater® reagent is an aqueous, non-toxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA through RNase inactivation. RNAstable® reagent is used to coat the bottom of storage tubes or wells, protecting picogram to microgram amounts of RNA at room temperature.

Related Resources

Related Webinars

Extracting high quality RNA and active protein from a single homogenate. Benefits of our Novel Stabilyser™ reagent. Author Natasha L. Pirman, PhD, Lead Application Scientist, Molecular Workflow Tools.
RNA Extraction and Purification

Watch our brief webinar to learn how the Stabilyser™ reagent enables efficient tissue lysis and stabilization of high-quality RNA and functionally active protein.

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Learn the basics of DNA and RNA gel electrophoresis, and how these techniques can be used to separate and purify DNA and RNA molecules based on size and charge for cloning, PCR, mass spectrometry, next generation sequencing (NGS), and Northern and Southern blotting applications.
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Quantitative PCR (qPCR) or quantitative Real-Time PCR applications, utilizing fluorescent report molecules, suitable for your microRNA, genotyping, and gene expression analyses.
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