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Membrane Immunodetection Devices

Western blotting immunodetection involves the use of antibodies to detect target protein. Blotted membranes containing transferred proteins are blocked, followed by antibody incubation and detection using chemiluminescence, colorimetric, or fluorescent methods. Membrane immunodetection devices facilitate the processing of Western blots by simplifying workflows and accelerating detection times. 

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The Snap I.D. 2.0 protein detection system, showcasing a sleek white base with two blue adjustment knobs and a viewing screen, alongside designated areas for sample placement, embodying modern protein analysis technology

SNAP i.d.® 2.0 Protein Detection System

The SNAP i.d.® 2.0 Protein Detection System is a unique, vacuum-driven system that pulls reagents through the membrane, increasing membrane contact while eliminating dependence on diffusion or agitation. This improvement in three-dimensional reagent distribution decreases the length of time required for immunodetection. While traditional Western blotting requires 4 to 24 hours for the blocking, antibody incubation, and washing steps, the SNAP i.d.® 2.0 protocol takes only 30 minutes with no loss of signal intensity or reduction in blot quality. All immunodetection steps after protein transfer to a membrane (i.e., blocking, washing, and primary and secondary antibody incubations) can be performed with the SNAP i.d.® 2.0 System.


Related Resources

  • Protocol: Protein Blotting Handbook

    The seventh edition of the Protein Blotting Handbook offers comprehensive guidance on optimizing antibody concentrations and reducing background interference, along with expanded data and protocols for fluorescence detection. The handbook represents the collective experience of application scientists and technical service specialists.

  • Article: 30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System

    The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.

  • Brochure: Rethink Western Blotting

    Access to our Western blotting expertise is easy—flip to our troubleshooting section at the end of this brochure, or learn more.




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