Western blotting immunodetection involves the use of antibodies to detect target protein. Blotted membranes containing transferred proteins are blocked, followed by antibody incubation and detection using chemiluminescence, colorimetric, or fluorescent methods. Membrane immunodetection devices facilitate the processing of Western blots by simplifying workflows and accelerating detection times.
Immobilon® GO is a hands-free immunodetection device designed for mini Western blot processing. The unique design uses continuous, sequential fluid convection across a flow matrix to enable autonomous antibody binding and membrane wash steps during immunodetection. Immobilon® GO is intended for one-time use and does not require an external power or vacuum source for operation. Western blots can be processed in as little as 3 hours or may remain in the device overnight for processing.
The SNAP i.d.® 2.0 Protein Detection System is a unique, vacuum-driven system that pulls reagents through the membrane, increasing membrane contact while eliminating dependence on diffusion or agitation. This improvement in three-dimensional reagent distribution decreases the length of time required for immunodetection. While traditional Western blotting requires 4 to 24 hours for the blocking, antibody incubation, and washing steps, the SNAP i.d.® 2.0 protocol takes only 30 minutes with no loss of signal intensity or reduction in blot quality. All immunodetection steps after protein transfer to a membrane (i.e., blocking, washing, and primary and secondary antibody incubations) can be performed with the SNAP i.d.® 2.0 System.