Polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE are common techniques used for protein separation. Protein gels can be hand-casted or purchased as pre-cast gels for convenience. The percentage of acrylamide in the gel affects resolution of protein bands, with higher percentages of acrylamide useful for resolving low molecular weight proteins and lower percentages of acrylamide useful in resolving higher molecular weight protein bands. Gradient pre-cast gels cover a wider range of molecular weight sizes. Hand-casting gel reagents, pre-cast gels, and running buffer powders are available for your lab needs.
mPAGE® Bis-Tris precast gels provide excellent protein resolution at a budget-friendly price. Offering high resolution of protein bands, mPAGE® gels require shorter run times and are compatible with large sample volumes, making mPAGE® precast gels a valuable tool for protein research.
Cast your own polyacrylamide gels using TurboMix™ Bis-Tris kits and solutions. TurboMix™ Bis-Tris Gel Casting Kits eliminate the variability and time-consuming nature of hand casting gels by supplying pre-mixed buffer and acrylamide solutions.
Two buffers that can be used as running buffers for SDS-PAGE gels: MES and MOPS. MES has a lower pKa than MOPS, which enables faster run times. MES buffer gives better separation of proteins at lower molecular weights, while MOPS buffer provides better separation at higher molecular weights. MES and MOPS SDS buffer powders are available for fast and easy preparation of running buffer for protein gel electrophoresis.
PAGE and SDS-PAGE gels can be hand-casted using acrylamide/bisacrylamide with TEMED and ammonium persulfate to polymerize the gel. The reagents are prepared, mixed together, then poured between two glass plates to polymerize. Acrylamide and bisacrylamide are neurotoxins in solution, so care should be taken to avoid direct contact. High purity TEMED, ammonium persulfate, and bisacrylamide and acrylamide in powder or solution form are available for hand casting gels.
Article: How to Run an mPAGE® Protein Gel Using a Bio-Rad Electrophoresis System
Article: How to Run an mPAGE® Protein Gel Using an Invitrogen XCell Surelock® Mini-Cell Electrophoresis System
Protocol: Protein Blotting Handbook
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Article: Western Blotting Protocol (Immunoblotting Protocol)