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Protein Sample Prep

Sample preparation is critical to prepare and purify proteins for functional or structural studies. Our complete range of reagents and enzymes for cell lysis and protein extraction provide you with an array of options so that you can put together the perfect extraction protocol for your particular cells and protein. We offer a wide vareity of products for protein purification, including agarose resins, magnetic beads, and FLAG® tag technology. Our membrane-based, protein sample concentration technologies, including tangential flow filtration (TFF)-based separation, enable fast, easy concentration, desalting and buffer exchange.

Cell lysis for protein extraction.
Whole Cell Lysis Reagents & Enhancers

Protein extraction kits, cell lysis buffers, and reagents for solubilizing proteins from bacteria, yeast, and insect cultures, as well as plant and mammalian cell cultures and tissue samples.

Proteins in solution
Protease & Phosphatase Inhibitor Cocktails

Protease and phosphatase inhibitors and cocktails to prevent proteolysis and protein dephosphorylation, and to preserve the active state of proteins during cell lysis, protein extraction, and sample preparation.

Cell fractionation and protein enrichment in proteomics research
Subcellular Fractionation, Enrichment, and Depletion Reagents

Fully validated subcellular fractionation and protein enrichment and depletion kits tailored for proteomics research and downstream applications including activity assays, protein microarrays, immunoblotting, ELISA, 2D gel electrophoresis, and mass spectrometry.

Protein purification of recombinant proteins using affinity chromatography
Recombinant/Fusion Tag Protein Purification

Affinity purification resins and buffers for purifying His-tagged, GST-tagged, FLAG® tagged, S-tag™, Strep-Tag II, and T7-tagged proteins under native or denaturing conditions using batch or column purification techniques.

Cytiva HiTrap™ Protein G HP purification columns
Antibody Protein Purification

Antibody purification resins and prepacked columns for lab scale, process scale, and industry scale workflow

Biotinylated Protein Purification

Streptavidin and avidin resins and prepacked columns for small- to large-scale purification of biotinylated proteins and antibodies.

Protein purification of recombinant proteins using affinity chromatography
FLAG purification

Expression vectors, affinity resins, and antibodies for expressing, purifying, and detecting FLAG® tagged fusion proteins.

Principle for protein concentration by ultrafiltration: larger molecular weight protein is retained while excess liquid and lower molecular weight particles pass into the filtrate.
Laboratory Concentration Devices

Centrifugal and pressurized ultrafiltration devices for high recovery protein concentration and concentration of other biological and environmental samples in preparation for downstream applications.

Continuous diafiltration for buffer exchange, desalting, and dialysis
Laboratory Dialysis & Diafiltration Devices

Dialyzers, centrifugal ultrafiltration devices, and stirred cells for buffer exchange, dialysis, desalting, and diafiltration of 10 µL – 1,200 mL sample volumes.

Amicon® Ultra centrifugal devices for protein concentration, desalting, and buffer exchange
Amicon® Devices

Centrifugal and pressurized ultrafiltration devices for protein concentration, desalting, buffer exchange, separation, and diafiltration of biological and environmental samples in preparation for downstream applications.

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