Monoclonal and polyclonal antibodies are commonly used in basic research and drug discovery. Antibody-based biologics also comprise a growing proportion of new therapeutics. Affinity chromatography using Protein A, Protein G, and Protein L crosslinked media plays a major role in the isolation and production of purified antibodies for lab scale, process scale, and industrial scale workflows.
Agarose beads and affinity gels enable antibody purification using Protein A or Protein G cross-linked to beaded agarose. Good for small to large scale purification, these resins are suitable for gravity flow column purification, low-speed centrifugation in batch processing, and low-pressure chromatography procedures. EZview™ affinity gels incorporate a red dye that allows the user to easily differentiate pellet from supernatant, resulting in less protein loss during batch purification. Immunoprecipitation kits include affinity gels, mini-spin columns, and lysis reagent and are specifically designed to allow maximal recovery of proteins from immunoprecipitates.
Fast Flow Sepharose™ and Fast flow agarose use highly crosslinked agarose beads or gels that can withstand higher pressures compared to standard agarose bead resins. This makes these resins suitable for fast protein liquid chromatography (FPLC) purification methods. These resins are recommended for medium to large scale antibody purification.
PureProteome™ Protein A, Protein G, and Protein A/G magnetic beads provide a rapid and reproducible way to purify immunoglobulins from complex mixtures such as serum, plasma or cell culture supernatant samples. Antibodies are purified using a magnetic rack or platform to separate beads from wash and elution fractions. This procedure is suitable for low throughput using single microfuge tubes or higher throughput using automation.
A wide range of Cytiva™ Amersham chromatography media are available for purification of monoclonal and polyclonal antibodies from different sources.
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