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Subcellular Fractionation, Enrichment, and Depletion Reagents

Subcellular fractionation techniques

Subcellular fractionation and protein enrichment enable the identification and study of proteins in proteomics research. A wide selection of kits for processing samples from cells, tissues, bacteria, plants, and other sample types is available for subcellular fractionation, protein extraction, depletion, and enrichment.

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kits (11)

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Sigma-Aldrich (9)

dry powder (1)
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ProteoExtract<SUP>®</SUP> Subcellular Proteome Extraction Kit
539790

ProteoExtract® Subcellular Proteome Extraction Kit

Mitochondria Isolation Kit
MITOISO2

Mitochondria Isolation Kit

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

Mitochondria Isolation Kit
MITOISO1

Mitochondria Isolation Kit

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

Endoplasmic Reticulum Isolation Kit
ER0100

Endoplasmic Reticulum Isolation Kit

isolation of intact ER from mammalian soft tissues and cultured cells

ProteoPrep<SUP>®</SUP> Immunoaffinity Albumin & IgG Depletion Kit
PROTIA

ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit

Lysosome Isolation Kit
LYSISO1

Lysosome Isolation Kit

sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells

Chloroplast Isolation Kit
CPISO

Chloroplast Isolation Kit

isolation of intact chloroplasts from leaves

ProteoExtract<SUP>®</SUP> Transmembrane Protein Extraction Kit
71772

ProteoExtract® Transmembrane Protein Extraction Kit

Peroxisome Isolation Kit
PEROX1

Peroxisome Isolation Kit

isolate peroxisomes from tissues and cells

ProteoPrep<SUP>®</SUP> Blue Albumin & IgG Depletion Kit
PROTBA

ProteoPrep® Blue Albumin & IgG Depletion Kit

ProteoPrep<SUP>®</SUP> Membrane Extraction Kit
PROTMEM

ProteoPrep® Membrane Extraction Kit

Mammalian Cell Lysis Kit
MCL1

Mammalian Cell Lysis Kit

Golgi Isolation Kit
GL0010

Golgi Isolation Kit

sufficient for 50 g (tissue)

ProteoPrep<SUP>®</SUP> Universal Extraction Kit
PROTTWO

ProteoPrep® Universal Extraction Kit

ProteoExtract<SUP>®</SUP> Albumin/IgG Removal Kit, Maxi
122643

ProteoExtract® Albumin/IgG Removal Kit, Maxi

ProteoPrep<SUP>®</SUP> Detergent Sample Kit
PROTDT

ProteoPrep® Detergent Sample Kit

ProteoPrep<SUP>®</SUP> Reduction and Alkylation Kit
PROTRA

ProteoPrep® Reduction and Alkylation Kit

ProteoExtract<SUP>®</SUP> Tissue Dissociation Buffer Kit
539720

ProteoExtract® Tissue Dissociation Buffer Kit

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Organelle Isolation Kits

The biogenesis and maintenance of intracellular organelles is fundamental in cell biology. Establishing and maintaining order requires mechanisms to synthesize and localize proteins to specific organelles and monitor and regulate individual organelles. Targeted proteomics profiling can be partnered with protein pathway function by enriching for organelles and analyzing their contents. These kits and reagents enable researchers to enrich for functional mitochondria, chloroplasts and more with companion kits to determine organelle integrity.

  • Specially formulated extraction reagents and proven procedures suitable for small- or large-scale isolation
  • Isolate intact, functionally active organelles for protein extraction, in vitro assays, and other studies

ProteoExtract® Kits

The ProteoExtract® kits cover the different steps in proteomics sample preparation from protein extraction and abundant protein removal to concentration of protein mixtures, removal of interfering substances, digestion of proteins, selective capturing of phosphorylated peptides, and selective enrichment for specific protein classes. All kits are cross-compatible.

  • Efficient and reproducible protein extraction
  • Protease inhibitor cocktails improve results in downstream analyses
  • Better spot resolution facilitated by nucleic acid digestion with protease-free Benzonase® nuclease
  • Designed for compatibility with many applications including activity assays, Western blots, 1D and 2D PAGE, and mass spectrometry
  • Optimized protocols for different biological samples

ProteoPrep® Extraction and Depletion Kits

ProteoPrep® kits and individual extraction reagents allow for selective or total protein extracts from cellular samples. The protein extractions obtained with each component can be optimized to meet your individual needs. The reducing and alkylating reagents produce protein samples that exhibit improved focusing and decreased streaking in 2D gels. Each kit reagent is also available as an individual product for researchers who have optimized an extraction protocol using one chaotropic extraction reagent.

  • Innovative detergent preparations
  • Premixed solubilization solutions

Related Resources

Related Product Categories
Cell Lysis Buffers & Protein Extraction Reagents
Protein extraction kits, cell lysis buffers, and reagents for solubilizing proteins from bacteria, yeast, and insect cultures, as well as plant and mammalian cell cultures and tissue samples.
Protein Mass Spectrometry
To meet your mass spectrometry needs, explore our comprehensive offering of kits, tagging reagents, calibrants, and MS standards.
Phosphatase & Protease Inhibitors
Inhibitors and cocktails preserve protein activity during cell lysis and sample prep, preventing proteolysis and dephosphorylation.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Protein Gel Electrophoresis and Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
Related Applications
Lysis & Protein Extraction
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
Protein Purification
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Protein Expression
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
Protein Mass Spectrometry
An overview of techniques for protein mass spectrometry used in the identification and quantification of proteins, post-translational modifications, glycan profiling, and protein-protein interactions in drug discovery and proteomics research.
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