Collagenase
Collagenase is an endopeptidase that effectively breaks down the collagen at its peptide bond regions under physiological pH & temperature conditions. Being a protein, it is highly sensitive to pH, temperature, and several other factors that can lead to protein denaturation. The activity, conformation, and properties of collagenase are highly influenced by external factors.
Collagenase consists of different types, such as I, II, III, IV, V, VI VII, VIII, XI, and a special collagenase for liver cells. Collagenase from Clostridium histolyticum is classified into 6 groups, namely, viz, alpha, beta, gamma, delta, epsilon, and zeta. It serves as an important catalyst and finds applications in stem cell biology, cancer research, and immunology.
Products
Collagenase D
from Clostridium histolyticum
Collagenase from Clostridium histolyticum
suitable for release of physiologically active rat hepatocytes, Type IV, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
Collagenase A
from Clostridium histolyticum
Collagenase from Clostridium histolyticum
for general use, Type I, ≥125 CDU/mg solid
Collagenase P
from Clostridium histolyticum
Collagenase/Dispase®
lyophilized, non-sterile, optimum pH 7.0-8.0
Collagenase from Clostridium histolyticum
suitable for release of physiologically active rat epididymal adipocytes, Type II, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
Collagenase from Clostridium histolyticum
Type IA, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid, For general use
Collagenase from Clostridium histolyticum
suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV), Type VIII, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
Collagenase from Clostridium histolyticum
Type XI, 2-5 FALGPA units/mg solid, ≥800 CDU/mg solid
Collagenase from Clostridium histolyticum
Type V, ≥1 FALGPA units/mg solid, >125 CDU/mg solid
Collagenase type I
The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.
Collagenase B
from Clostridium histolyticum
Collagenase from Clostridium histolyticum
lyophilized powder, ≥125 CDU/mg solid (CDU = collagen digestion units), 0.5-5.0 FALGPA units/mg solid
Collagenase from Clostridium histolyticum
powder, Suitable for the digestion and isolation of physiologically active pancreatic islet cells, suitable for cell culture
Collagenase Type II, Cls II
Collagenase Type IV, Cls IV
Collagenase from Clostridium histolyticum
lyophilized powder (from 0.2μm filtered solution), 0.5-5.0 FALGPA units/mg solid, suitable for cell culture
Collagenase from Clostridium histolyticum
lyophilized powder (from 0.2 μm filtered solution), suitable for cell culture
Collagenase from Clostridium histolyticum
high purity, purified by chromatography, Type VII, ≥4 FALGPA units/mg solid, lyophilized powder, ≥700 CDU/mg solid (CDU = collagen digestion units)
Collagenase for cell/tissue isolation
Collagenase supports a wide range of research as it can be used to isolate cells/tissues by degrading collagen in the skin, blood vessels, tendons, and bones. The type of collagenase is chosen based on the specific type of cell or tissue that needs to be isolated. Collagenase type I is suitable for the isolation of fat, lung, epithelial, and adrenal tissue cells. Collagenase type II is ideal for the separation of the liver, thyroid, bone, heart, and salivary gland tissues. Collagenase type IV can break down a variety of tissues, and type V is applicable in the isolation of pancreatic islet tissues by separating the connective tissues into single cells. Collagenase disaggregation is ideal for culturing mouse kidneys, human adult, and fetal brains.
Collagenase for cell culture
Collagenase is gentle, requires no agitation, and dissociates well at optimum temperature and pH. Therefore, it is ideal for culturing mouse kidneys, human adult and fetal brains, human tumors, and epithelial tissues.
Collagenase as a therapeutic
Collagenase plays an important role in various clinical applications, including the treatment of lumbar disc and sciatic nerve issues, trauma therapy, rheumatoid arthritis, and osteoarthritis.
Related Resources
- Tissue Dissociation Guide: Collagenase, Dispase, and Liberase Enzyme Types
Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.
- Core Cell Culture
Cell culture optimization relies on well-characterized reagents and high-quality products for consistent conditions.
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