Evidence is emerging that estrogen receptor alpha (ERalpha) is central to the rapid transduction of estrogen signaling to the downstream kinase cascades; however, the mechanisms underlying this nongenomic function are not fully understood. Here we report a paradigm of ERalpha
Journal of cell science, 125(Pt 7), 1814-1826 (2012-02-14)
Neural precursor expressed, developmentally down-regulated 9 (NEDD9), a member of the Cas family of signal transduction molecules, is amplified at the genetic level in melanoma, and elevated expression levels have been shown to correlate with melanoma progression and metastasis. NEDD9
The actin nodule is a novel F-actin structure present in platelets during early spreading. However, only limited detail is known regarding nodule organization and function. Here we use electron microscopy, SIM and dSTORM super-resolution, and live-cell TIRF microscopy to characterize
Localization and identification of sumoylated proteins in human sperm: excessive sumoylation is a marker of defective spermatozoa.
Vigodner, M; Shrivastava, V; Gutstein, LE; Schneider, J; Nieves, E; Goldstein et al.
Beside their established function in shaping cell architecture, some cell polarity proteins were proposed to participate to lymphocyte migration, homing, scanning, as well as activation following antigen receptor stimulation. Although PALS1 is a central component of the cell polarity network
Digital microfluidic assay for protein detection.
Mok, J; Mindrinos, MN; Davis, RW; Javanmard, M
Proceedings of the National Academy of Sciences of the USA null
Neuromuscular junction (NMJ) formation requires agrin, a factor released from motoneurons, and MuSK, a transmembrane tyrosine kinase that is activated by agrin. However, how signal is transduced from agrin to MuSK remains unclear. We report that LRP4, a low-density lipoprotein
MicroRNAs (miRNAs) carry out post-transcriptional control of a multitude of cellular processes. Aberrant expression of miRNA can lead to diseases, including cancer. Gliomas are aggressive brain tumors that are thought to arise from transformed glioma-initiating neural stem cells (giNSCs). With
Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBP? expression and contributes to transformation of breast tumor cells.
Sarkar, TR; Sharan, S; Wang, J; Pawar, SA; Cantwell, CA; Johnson, PF; Morrison, DK; Wang et al.
Developmental and comparative immunology, 36(1), 216-221 (2011-09-03)
The pituitary hormone prolactin (PRL) is a multifunctional polypeptide which act as a key component of the neuroendocrine-immune loop and as a local regulator of the macrophage response. The involvement of PRL in regulating monocyte/macrophage functions is suggested by the
Copper (II) oxide (CuO) nanoparticles (NP) are widely used in industry and medicine. In our study we evaluated the response of BEAS-2B human lung cells to CuO NP, using Stable isotope labeling by amino acids in cell culture (SILAC)-based proteomics
Angiopoietin-1/Tie-2 activation contributes to vascular survival and tumor growth during VEGF blockade.
Cells must make appropriate fate decisions within a complex and dynamic environment. In vitro studies indicate that the cytoskeleton acts as an integrative platform for this environmental input. External signals regulate cytoskeletal dynamics and the cytoskeleton reciprocally modulates signal transduction.
Heterogeneous kinetics of AKT signaling in individual cells are accounted for by variable protein concentration.
Journal of immunology (Baltimore, Md. : 1950), 186(2), 784-790 (2010-12-15)
Despite the defined function of the β-catenin pathway in thymocytes, its functional role in peripheral T cells is poorly understood. We report that in a mouse model, β-catenin protein is constitutively degraded in peripheral T cells. Introduction of stabilized β-catenin
In the mammalian embryo, few mechanical signals have been identified to influence organ development and function. Here, we report that an increase in the volume of interstitial or extracellular fluid mechanically induces growth of an organ system, that is, the
Journal of the National Cancer Institute, 104(23), 1815-1824 (2012-10-24)
Companion diagnostic tests can depend on accurate measurement of protein expression in tissues. Preanalytic variables, especially cold ischemic time (time from tissue removal to fixation in formalin) can affect the measurement and may cause false-negative results. We examined 23 proteins
Molecular and cellular biology, 29(7), 1933-1943 (2009-01-14)
The Src-associated substrate during mitosis with a molecular mass of 68 kDa (Sam68) is predominantly nuclear and is known to associate with proteins containing the Src homology 3 (SH3) and SH2 domains. Although Sam68 is a Src substrate, little is
Acquisition of a potent and selective TC-PTP inhibitor via a stepwise fluorophore-tagged combinatorial synthesis and screening strategy.
Clinical cancer research : an official journal of the American Association for Cancer Research, 17(9), 2679-2692 (2011-02-11)
The chromosomal deletion 11q affects biology and clinical outcome in chronic lymphocytic leukemia (CLL) but del11q-deregulated genes remain incompletely characterized. We have employed integrated genomic profiling approaches on CLL cases with and without del11q to identify 11q-relevant genes. We have
American journal of physiology. Regulatory, integrative and comparative physiology, 307(8), R945-R955 (2014-08-01)
Transient receptor potential vanilloid family type 4 (TRPV4) channels are expressed in central neuroendocrine neurons and have been shown to be polymodal in other systems. We previously reported that in the rodent, a model of dilutional hyponatremia associated with hepatic
The Journal of clinical investigation, 123(12), 5190-5202 (2013-11-10)
Myasthenia gravis (MG) is the most common disorder affecting the neuromuscular junction (NMJ). MG is frequently caused by autoantibodies against acetylcholine receptor (AChR) and a kinase critical for NMJ formation, MuSK; however, a proportion of MG patients are double-negative for
Mer or Axl receptor tyrosine kinase inhibition promotes apoptosis, blocks growth and enhances chemosensitivity of human non-small cell lung cancer.
Linger, RM; Cohen, RA; Cummings, CT; Sather, S; Migdall-Wilson, J; Middleton, DH; Lu et al.
Prolactin-induced Jak2 phosphorylation of RUSH: a key element in Jak/RUSH signaling.
Journal of molecular medicine (Berlin, Germany), 87(2), 139-144 (2008-12-17)
Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1) inhibits insulin-receptor (IR) signaling and, when over-expressed, induces insulin resistance in vitro and in vivo. Understanding the regulation of ENPP1 expression may, thus, unravel new molecular mechanisms of insulin resistance. Recent data point to a