Chemistry (Weinheim an der Bergstrasse, Germany), 16(1), 158-166 (2009-12-02)
Fluorescent markers emitting in the red are extremely valuable in biological microscopy since they minimize cellular autofluorescence and increase flexibility in multicolor experiments. Novel rhodamine dyes excitable with 630 nm laser light and emitting at around 660 nm have been...
Fast, super resolution imaging via Bessel-beam stimulated emission depletion microscopy.
Zhang, P.; Goodwin, P.M.; Werner, J.H.
Optics Express, 22(10), 12398-12409 (2014)
Maturation of active zone assembly by Drosophila Bruchpilot.
Fouquet, W.; et al.
The Journal of Cell Biology, 186(1), 129-145 (2009)
Spatial organization of proteins in metastasizing cells.
Ronnlund, D.; et al.
Cytometry, 83(9), 855-865 (2013)
Block Copolymer Nanostructures Mapped by Far-Field Optics.
We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require...
Stimulated emission depletion (STED) microscopy usually employs a scanning excitation beam that is superimposed by a donut-shaped STED beam for keeping the fluorophores at the periphery of the excitation spot dark. Here, we introduce a simple birefringent device that produces...
We report on a straightforward yet powerful implementation of stimulated emission depletion (STED) fluorescence microscopy providing subdiffraction resolution in the far-field. Utilizing the same super-continuum pulsed laser source both for excitation and STED, this implementation of STED microscopy avoids elaborate...
Nanoscale organization of nicotinic aceylcholine receptors by stimulated emission depletion microscopy.
Kellner, R.R., et al.
Neuroscience, 144(1), 135-143 (2007)
Two-photon excitation STED microscopy
Moneron, G.; Hell, S. W.
Optics Express, 17(17), 14567-14573 (2009)
STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA.
We report stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams. Lateral fluorescence confinement from the scanning focal spot delivered a resolution of 29-60 nm in the focal plane, corresponding to a 5-8-fold improvement over the diffraction...
Experimental Proof of Concept of Nanoparticle-Assisted STED.
Sonnefraud, Y.; et al
Nano Letters, 14(8), 4449-4453 (2014)
A novel nanoscopic tool by combining AFM with STED microscopy.
Harke, B.; et al
Optical Nanoscopy, 1(1), 3-3 (2012)
STED imaging of green fluorescent nanodiamonds containing nitrogen-vacancy-nitrogen centers.
Laporte, G.; Psaltis, D.
Biomedical Optics Express, 7(1), 34-44 (2016)
Self-Calibrated Line-Scan STED-FCS to Quantify Lipid Dynamics in Model and Cell Membranes.
Benda, A.; Ma, Y.; Gaus, K.
Biophysical Journal, 108(3), 596-609 (2015)
Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles.
Chiang, HC.; et al
Nature Communications, 5, 3356-3356 (2014)
Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy.
Steshenko, O.; et al.
Biophysical Journal, 110(11), 2441-2450 (2016)
Naked Dense Bodies Provoke Depression.
Hallermann, S.; et al.
The Journal of Neuroscience, 30(43), 14340-14345 (2010)
Comparing video-rate STED nanoscopy and confocal microscopy of living neurons.
Lauterbach, M. A.; et al.
Journal of Biophotonics, 3(7), 417-424 (2010)
Resolving the structure of inner ear ribbon synapses with STED microscopy.
Synapse, 69(5), 242-255 (2015)
STED with wavelengths closer to the emission maximum.
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