Chemistry (Weinheim an der Bergstrasse, Germany), 16(1), 158-166 (2009-12-02)
Fluorescent markers emitting in the red are extremely valuable in biological microscopy since they minimize cellular autofluorescence and increase flexibility in multicolor experiments. Novel rhodamine dyes excitable with 630 nm laser light and emitting at around 660 nm have been...
We report on a straightforward yet powerful implementation of stimulated emission depletion (STED) fluorescence microscopy providing subdiffraction resolution in the far-field. Utilizing the same super-continuum pulsed laser source both for excitation and STED, this implementation of STED microscopy avoids elaborate...
Size-Dependent Localization and Quantitative Evaluation of the Intracellular Migration of Silica Nanoparticles in Caco-2 Cells.
Schubbe, S.; et al.
Chemistry of Materials, 24(5), 914-923 (2012)
2PE-STED Microscopy with a Single Ti. Sapphire Laser for Reduced Illumination.
Li, Q.; Wang, Y.; et al
PLoS ONE, 9(2), e88464-e88464 (2014)
Sub-diffraction imaging of nitrogen-vacancy centers in diamond by stimulated emission depletion and structured illumination
Xusan, Y., et al.
Royal Society of Chemistry Advances, 4(22), 11305-11305 (2014)
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis.
Willig K.I.; et al.
Nature, 440(7086), 935-939 (2006)
A novel nanoscopic tool by combining AFM with STED microscopy.
Harke, B.; et al
Optical Nanoscopy, 1(1), 3-3 (2012)
STED imaging of green fluorescent nanodiamonds containing nitrogen-vacancy-nitrogen centers.
Laporte, G.; Psaltis, D.
Biomedical Optics Express, 7(1), 34-44 (2016)
Self-Calibrated Line-Scan STED-FCS to Quantify Lipid Dynamics in Model and Cell Membranes.
Benda, A.; Ma, Y.; Gaus, K.
Biophysical Journal, 108(3), 596-609 (2015)
Sub-Diffraction Nano Manipulation Using STED AFM.
Chacko, Jenu V.; et al.
PLoS ONE, 8(6), e66608-e66608 (2013)
Experimental Proof of Concept of Nanoparticle-Assisted STED.
Sonnefraud, Y.; et al
Nano Letters, 14(8), 4449-4453 (2014)
Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy.
Steshenko, O.; et al.
Biophysical Journal, 110(11), 2441-2450 (2016)
Spatial organization of proteins in metastasizing cells.
Ronnlund, D.; et al.
Cytometry, 83(9), 855-865 (2013)
Long working distance fluorescence lifetime imaging with stimulated emission and electronic time delay.
We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require...
STED microscopy resolves nanoparticle assemblies.
Willig K.I.; et al.
New Journal of Physics, 8(6), 106-106 (2006)
Block Copolymer Nanostructures Mapped by Far-Field Optics.
Ullal, C.K.; et al
Nano Letters, 9(6), 2497-2500 (2009)
Frequency dependent detection in a STED microscope using modulated excitation light.
We report stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams. Lateral fluorescence confinement from the scanning focal spot delivered a resolution of 29-60 nm in the focal plane, corresponding to a 5-8-fold improvement over the diffraction...
Nanoscale organization of nicotinic aceylcholine receptors by stimulated emission depletion microscopy.
Kellner, R.R., et al.
Neuroscience, 144(1), 135-143 (2007)
Naked Dense Bodies Provoke Depression.
Hallermann, S.; et al.
The Journal of Neuroscience, 30(43), 14340-14345 (2010)
Stimulated emission depletion (STED) microscopy usually employs a scanning excitation beam that is superimposed by a donut-shaped STED beam for keeping the fluorophores at the periphery of the excitation spot dark. Here, we introduce a simple birefringent device that produces...
STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA.
Heller, I.; et al.
Nature Methods, 10(9), 910-916 (2013)
Comparing video-rate STED nanoscopy and confocal microscopy of living neurons.
Lauterbach, M. A.; et al.
Journal of Biophotonics, 3(7), 417-424 (2010)
Two-photon excitation STED microscopy
Moneron, G.; Hell, S. W.
Optics Express, 17(17), 14567-14573 (2009)
Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles.
Chemistry (Weinheim an der Bergstrasse, Germany), 18(41), 12986-12998 (2012-09-13)
Fluorescent dyes emitting red light are frequently used in conventional and super-resolution microscopy of biological samples, although the variety of the useful dyes is limited. We describe the synthesis of rhodamine-based fluorescent dyes with absorption and emission maxima in the...
Two-photon excitation STED microscopy by utilizing transmissive liquid crystal devices.
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