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Fluoreszierende Rhodamine und fluorogene Carbopyronine fur die STED-Mikroskopie lebender Zellen.
Butkevich, Alexey N.; et al.
Angewandte Chemie (International Edition in English), 128(10), 3350-3355 (2016)
STED microscopy to monitor agglomeration of silica particles inside A549 cells.
Schubbe, S., et al.
Advanced Engineering Materials, 12, 417-422 (2010)
Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells.
Stagge, F.; et al.
PLoS ONE, 8(10), e78745-e78745 (2013)
Experimental investigations on fluorescence excitation and depletion of ATTO 390 dye.
Luo, D., et al.
Optics & Laser technology, 45, 723-725 (2013)
An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics.
Vicidomini, G.; et al.
Nano Letters, 15(9), 5912-5918 (2015)
A novel nanoscopic tool by combining AFM with STED microscopy.
Harke, B., et al.
Optical Nanoscopy, 1, 3-3 (2012)
Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells.
Hedde P.N.; et al.
Nature Communications, 4, 2093-2093 (2013)
Synaptic membrane proteins form stable microdomains in early endosomes.
Geumann, U.; et al.
Microscopy Research and Technique, 73(6), 606-617 (2010)
Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom STED microscope.
Meyer, S. A.; Ozbay, B.
Proceedings of SPIE, 8950, 89500-89500 (2014)
Enhancing stimulated emission-based fluorescence detection with interferometric setup.
Chung, S.-S.M.; Deng, J.-H.
Proceedings of SPIE, 8948, 89481-89481 (2014)
STED Microscopy to Monitor Agglomeration of Silica Particles Inside A549 Cells.
Schubbe, S.; Cavelius, C.
Advanced Energy Materials, 12, 417-422 (2010)
Quantification of Internalized Silica Nanoparticles via STED Microscopy.
Peuschel, H.; et al.
BioMed Research International, 2015(1), 1-16 (2015)
STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins.
Fitzpatrick, JA.; et al.
Bioconjugate Chemistry, 20(10), 1843-1847 (2009)
Far-field optical nanoscopy based on continuous wave laser stimulated emission depletion.
Kuang, C.; Zhao, W.; Wang, G.; et al.
The Review of Scientific Instruments, 81(5), 53709-53709 (2010)
Super-Resolution Imaging Reveals That AMPA Receptors Inside Synapses Are Dynamically Organized in Nanodomains Regulated by PSD95.
Nair D.; et al.
The Journal of Neuroscience, 33(32), 13204-13224 (2013)
Christian B Gerhold et al.
Nucleic acids research, 40(21), 11036-11046 (2012-09-15)
Nuclear actin-related proteins (Arps) are subunits of several chromatin remodelers, but their molecular functions within these complexes are unclear. We report the crystal structure of the INO80 complex subunit Arp8 in its ATP-bound form. Human Arp8 has several insertions in...
Exploring single-molecule dynamics with fluorescence nanoscopy.
Ringemann, Ch.; et al
New Journal of Physics, 11(10), 103054-103054 (2009)
Munc18-1 Tuning of Vesicle Merger and Fusion Pore Properties.
Jorgacevski, J.; et al.
The Journal of Neuroscience, 31(24), 9055-9066 (2011)
SNARE Function Is Not Involved in Early Endosome Docking.
Geumann, U.; et al.
Molecular Biology of the Cell, 19(12), 5327-5337 (2008)
Marisa L Martin-Fernandez et al.
International journal of molecular sciences, 13(11), 14742-14765 (2012-12-04)
Insights from single-molecule tracking in mammalian cells have the potential to greatly contribute to our understanding of the dynamic behavior of many protein families and networks which are key therapeutic targets of the pharmaceutical industry. This is particularly so at...
Volker Westphal et al.
Science (New York, N.Y.), 320(5873), 246-249 (2008-02-23)
We present video-rate (28 frames per second) far-field optical imaging with a focal spot size of 62 nanometers in living cells. Fluorescently labeled synaptic vesicles inside the axons of cultured neurons were recorded with stimulated emission depletion (STED) microscopy in...
Annedore Punge et al.
Microscopy research and technique, 71(9), 644-650 (2008-06-03)
Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100...
Resolving the structure of inner ear ribbon synapses with STED microscopy.
Rutherford, M.A.
Synapse, 69(5), 242-255 (2015)
Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature.
Eggeling, C.; et al.
Nature, 457, 1159-1162 (2009)
Kirill Kolmakov et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 18(41), 12986-12998 (2012-09-13)
Fluorescent dyes emitting red light are frequently used in conventional and super-resolution microscopy of biological samples, although the variety of the useful dyes is limited. We describe the synthesis of rhodamine-based fluorescent dyes with absorption and emission maxima in the...
Superresolution and Fluorescence Dynamics Evidence Reveal That Intact Liposomes Do Not Cross the Human Skin Barrier.
Dreier, J.; S?rensen, J. A.; Brewer, J. R.
PLoS ONE, 11(1) (2016)
Silvia Zorrilla et al.
Biochemistry, 46(51), 14996-15008 (2007-12-07)
CggR is the transcriptional repressor of the gapA operon encoding central glycolytic enzymes in Bacillus subtilis. Recently, a detailed mechanistic characterization of gapA induction revealed that the binding of fructose-1,6-bisphosphate (FBP) to a low affinity site on CggR (Kd >...
S E D Webb et al.
Optics express, 16(25), 20258-20265 (2008-12-10)
We combine single molecule fluorescence orientation imaging with single-pair fluorescence resonance energy transfer microscopy, using a total internal reflection microscope. We show how angles and FRET efficiencies can be determined for membrane proteins at the single molecule level and provide...
Two-photon excitation STED microscopy by utilizing transmissive liquid crystal devices.
Otomo, K.; et al.
Optics Express, 22(23), 28215-28215 (2014)
Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission.
Ge, J.; Kuang, C.; Lee, SS.; Kao, FJ
Optics Express, 20(27), 28216-28216 (2012)
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