Showing 1-30 of 255 results for "

09830

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Øystein Skjærvø et al.
The Analyst, 142(20), 3837-3847 (2017-09-13)
A concept integrating sampling and protein digestion is introduced here combining fast and simple fabrication by wax printing on filter paper with trypsin immobilized polymer beads. The paper reactors showed promising results with a high degree of protein digestion within...
Abby Sewell et al.
Molecular vision, 20, 1182-1191 (2014-09-16)
To compare the proteomic profile of a clinical isolate of Pseudomonas aeruginosa (P. aeruginosa) obtained from an infected cornea of a contact lens wearer and the laboratory strain P. aeruginosa ATCC 10145. Antibiotic sensitivity, motility, biofilm formation, and virulence tests...
Yu Jin Oh et al.
Journal of controlled release : official journal of the Controlled Release Society, 150(1), 56-62 (2010-11-13)
Inhaling corticosteroids, such as budesonide (BD), is the most common treatment for asthma. However, frequent steroid administration is associated with many side effects. We hypothesized that porous microparticles containing BD could provide an effective treatment method for asthma, as the...
Kavya Mohan et al.
PloS one, 9(6), e96551-e96551 (2014-06-10)
We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system and is capable of...
Victoria Pando-Robles et al.
Journal of proteomics, 111, 16-29 (2014-07-11)
Dengue is an important and growing public health problem worldwide with an estimated 100million new clinical cases annually. Currently, no licensed drug or vaccine is available. During natural infection in humans, liver cells constitute one of the main targets of...
V Mistretta et al.
Acta clinica Belgica, 69(1), 53-61 (2014-03-19)
Azole antifungals are a group of fungistatic agents that can be administered orally or parenterally. The determination of the concentrations of these antifungals (miconazole, fluconazole, ketoconazole, posaconazole, voriconazole, itraconazole, and its major active metabolite, hydroxy-itraconazole) in serum can be useful...
Jonathan Ashby et al.
ACS applied materials & interfaces, 6(17), 15412-15419 (2014-08-22)
Nanoparticles (NPs) adsorb proteins when in the biological matrix, and the resulted protein corona could affect NP-cell interactions. The corona has a dynamic nature with the adsorbed proteins constantly exchanging with the free proteins in the matrix at various rates....
Yan Liu et al.
Science translational medicine, 6(266), 266ra170-266ra170 (2014-12-17)
Doxorubicin is a highly effective anticancer chemotherapy agent, but its use is limited by its cardiotoxicity. To develop a drug that prevents this toxicity, we established a doxorubicin-induced cardiomyopathy model in zebrafish that recapitulates the cardiomyocyte apoptosis and contractility decline...
Nancy D Epsky et al.
Journal of economic entomology, 105(2), 471-479 (2012-05-23)
Tests were conducted that evaluated efficacy of wax matrix bait stations for Ceratitis capitata (Wiedemann) adults in Guatemala. Bait stations were exposed to outdoor conditions to determine effect of weathering on longevity as indicated by bait station age. Results of...
Chi-De Chen et al.
Journal of proteome research, 13(6), 2818-2829 (2014-05-03)
The ability to discriminate lung cancer malignant pleural effusion (LC-MPE) from benign pleural effusion has profound implications for the therapy and prognosis of lung cancer. Here, we established a pipeline to verify potential biomarkers for this purpose. In the discovery...
Lior Doron et al.
PloS one, 9(10), e111329-e111329 (2014-10-31)
Fusobacterium nucleatum is an oral anaerobe associated with periodontal disease, adverse pregnancy outcomes and colorectal carcinoma. A serine endopeptidase of 61-65 kDa capable of damaging host tissue and of inactivating immune effectors was detected previously in F. nucleatum. Here we...
Qichen Cao et al.
Analytical chemistry, 86(14), 6804-6811 (2014-06-11)
Core fucosylation (CF) is a special glycosylation pattern of proteins that has a strong relationship with cancer. The Food and Drug Administration (FDA) has approved the core fucosylated α-fetoprotein as a biomarker for the early diagnosis of hepatocellular carcinoma (HCC)....
Ming-Chuan Wang et al.
Analytical and bioanalytical chemistry, 407(5), 1343-1356 (2014-12-10)
Tyrosine phosphorylation is an important regulator of signaling in cellular pathways, and dysregulated tyrosine phosphorylation causes several diseases. Mass spectrometry has revealed the importance of global phosphoproteomic characterization. Analysis of tyrosine phosphorylation by studying the mass-spectrometry (MS)-determined phosphoproteome remains difficult...
Kélen Fabíola Arroteia et al.
PloS one, 9(8), e103566-e103566 (2014-08-02)
The epididymis has an important role in the maturation of sperm for fertilization, but little is known about the epididymal molecules involved in sperm modifications during this process. We have previously described the expression pattern for an antigen in epididymal...
Thea S B Kjeldsen et al.
The Journal of antimicrobial chemotherapy, 70(1), 62-70 (2014-09-04)
Knowledge about the regulatory mechanisms of CTX-M β-lactamase-encoding genes in Escherichia coli is limited. The objectives of this study were to determine the growth response of CTX-M-1-producing E. coli exposed to cefotaxime and to investigate how blaCTX-M-1 expression at mRNA...
Joanna A Majchrzykiewicz-Koehorst et al.
Journal of virological methods, 213, 75-83 (2014-12-17)
The rapid identification of existing and emerging respiratory viruses is crucial in combating outbreaks and epidemics. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid and reliable identification method in bacterial diagnostics, but has not been used in...
Dinh-Duy Pham et al.
International journal of pharmaceutics, 454(2), 668-677 (2013-04-23)
We have designed a novel formulation of pyrazinamide (PZA), an antitubercular drug within large porous particles intended for deep lung delivery. By simply spray-drying PZA, we have obtained crystalline particles of the δ polymorph of PZA that were unstable and...
Willy V Bienvenut et al.
Molecular systems biology, 16(7), e9464-e9464 (2020-07-08)
Protein acetylation is a highly frequent protein modification. However, comparatively little is known about its enzymatic machinery. N-α-acetylation (NTA) and ε-lysine acetylation (KA) are known to be catalyzed by distinct families of enzymes (NATs and KATs, respectively), although the possibility...
José Rivas et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(6), 7847-7865 (2020-04-18)
Transient receptor potential melastatin 4 (TRPM4) is a Ca2+ -activated nonselective cationic channel that regulates cell migration and contractility. Increased TRPM4 expression has been related to pathologies, in which cytoskeletal rearrangement and cell migration are altered, such as metastatic cancer....
John D Lapek et al.
Naunyn-Schmiedeberg's archives of pharmacology, 388(2), 161-173 (2014-11-07)
We have developed a method to identify previously undetected histidine and aspartic acid phosphorylations in a human prostate cancer progression model. A phosphoproteome of our cell line model is presented, with correlation of modified protein expression between the three states...
Nico P M Smit et al.
Journal of proteomics, 109, 143-161 (2014-06-28)
In this study, we have followed up on previous liquid chromatography (LC) multiple reaction monitoring (MRM) mass spectrometry (MS) approaches for measurement of apolipoprotein (apo) A-I and apo B100 in serum aiming for implementation of a multiplexed assay in a...
Teresa F Reyes et al.
Regulatory toxicology and pharmacology : RTP, 119, 104817-104817 (2020-11-11)
The production of soy leghemoglobin C2 (LegH) by Pichia pastoris (syn. K. phaffii) was developed by Impossible Foods to serve as a sustainable source of flavor and aroma in plant-based meats. The potential allergenicity and toxicity of a LegH from...
Chen Qian et al.
Huan jing ke xue= Huanjing kexue, 31(10), 2417-2422 (2011-01-15)
Air-drying is always accompanied by soil moisture loss. The different influences of air-drying on soil nitrification might due to the different nitrification responses to moisture changing of acid subtropical soils. So, after applying 0 and 150 mg x kg(-1) of...
Joshua J Klaene et al.
Journal of pharmaceutical sciences, 103(10), 3033-3042 (2014-07-22)
The formation of aspartyl succinimide is a common post-translational modification of protein pharmaceuticals under acidic conditions. We present a method to detect and quantitate succinimide in intact protein via hydrazine trapping and chemical derivatization. Succinimide, which is labile under typical...
A E-L Au et al.
Cell death & disease, 5, e1410-e1410 (2014-09-12)
Platelet activation is a frontline response to injury, not only essential for clot formation but also important for tissue repair. Indeed, the reparative influence of platelets has long been exploited therapeutically where application of platelet concentrates expedites wound recovery. Despite...
Tobias D Wheeler et al.
Analytical chemistry, 86(11), 5416-5424 (2014-05-03)
A high-throughput screening assay on a microfluidic chip was developed for the determination of charge variants of monocolonal antibodies (mAbs) in pI range of 7-10. This method utilizes microchip zone electrophoresis for rapid separation (<90 s) of mAb charge variants...
Shuaihua Zhang et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 906, 41-47 (2012-09-05)
A sensitive method for the determination of three cationic alkaloids (berberine, palmatine and jatrorrhizine) from human plasma samples was developed by micelle to solvent stacking (MSS) in capillary zone electrophoresis (CZE). In MSS, the sample preconcentration mainly relies on the...
Uta Greifenhagen et al.
Journal of proteome research, 14(2), 768-777 (2014-11-26)
Glycation refers to a nonenzymatic post-translational modification formed by the reaction of amino groups and reducing sugars. Consecutive oxidation and degradation can produce advanced glycation end products (AGEs), such as N(ε)-(carboxyethyl)lysine (CEL) and N(ε)-(carboxymethyl)lysine (CML). Although CEL and CML are...
Estela Giménez et al.
Analytica chimica acta, 866, 59-68 (2015-03-04)
In this work we demonstrate the potential of glycan reductive isotope labeling (GRIL) using [(12)C]- and [(13)C]-coded aniline and zwitterionic hydrophilic interaction capillary liquid chromatography electrospray mass spectrometry (μZIC-HILIC-ESI-MS) for relative quantitation of glycosylation variants in selected glycoproteins present in...
Pete Gagnon et al.
Journal of chromatography. A, 1374, 145-155 (2014-12-08)
Chromatin heteroaggregates comprising nucleosomal arrays, DNA, histone and non-histone host cell proteins contributed 28% of the host contaminant mass in an IgG cell culture harvest. A subset comprising soluble particles of 50-400nm was retained through its histone component by an...