Mycoplasma in broth (++++: 104
cfu/ml, +++ � 103-104 cfu/ml, ++: 102-103 cfu/ml, +: 10-102 cfu/ml). Mycoplasma PCR
ELISA was performed as described in Chapter 2, without prior enrichment. Samples pro-
with a 103 Reaction Buffer
available with and without MgCl2. The 103 Buffer without MgCl2
includes a separate tube of 25 mM MgCl2 for optimization.
Components: JumpStart Taq DNA Polymerase
103 PCR Buffer
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