105-40-8
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Process Guidance - Cellvento BHK-200
80
60
40
20
0
100
95
90
85
80
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ia
bi
lit
y
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)
0 8 16 24 32 40 48 56 64 72 80
Product Information Sheet - BPQ96
0.5-1 x 105 cells in 24-well plate or on cover slips and let grow overnight.
2. Dilute 4 to 8 µg of protein in 40 µl of HBS (IgG) or PBS (β-Galactosidase)
3. Hydrate BioPORTER dry film with 40 µl
Product Information Sheet - BPQ24
0.5-1 x 105 cells in 24-well plate or on cover slips and let grow overnight.
2. Dilute 4 to 8 µg of protein in 40 µl of HBS (IgG) or PBS (β-Galactosidase)
3. Hydrate BioPORTER dry film with 40 µl
User_Guide
temperature:
Milligard® Media: ≤ 20 mg ≤ 50 mg
Milligard® LPB Media:
0.2 μm ≤ 40 mg ≤ 100 mg
0.5 μm ≤ 40 mg ≤ 105 mg
1.2 μm ≤ 100 mg ≤ 250 mg
0.5/0.2 μm ≤ 80 mg ≤
Blood Agar Base No. 2, 110328 MIBIO
ATCC 25923 103-105 � 70 β -
Streptococcus pyogenes ATCC 19615 103-105 � 70 β +
Streptococcus pneumoniae
ATCC 6305
103-105 � 70 α -
Streptococcus agalactiae
ATCC 13813
103-105 � 70 - -
Listeria
Process Guidance Cellvento™ BHK-200 Medium EMDM
+ 10% DMSO
– Continue with step 8.
8th adaptation of high density culture in Cellvento™ BHK-200 medium
• Subculture the cells at a seeding density of 6×105 viable cells/mL in Cellvento™ BHK-
Stability study of orally disintegrating tablets (ODT)
39120454615221
104
125
105
120
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132
Ta
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et
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is
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gr
at
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[s
]
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The Effective Use of Protein Kinase Inhibitors
91 106 98 65 104 73 102 96 95 78 98 105 60 75 86
69 15 20 86 82 69 114 115 27 46 105 109 99 44
54 92 4 5 5 29 23 5
MILLIPLEX®MAP Human Myokine Panel is an optimized quantitative immunoassay that simultaneously measures 15 novel muscle-secreted factors
111 22 70 6156 38 44 166 34
std
7
26 18 26 20 115 58 35 111 24 105 8090 39 37 174 33
26 21 26 22 124 55 33 110 26 105 8025 40
The Effective Use of Protein Kinase Inhibitors
91 106 98 65 104 73 102 96 95 78 98 105 60 75 86
69 15 20 86 82 69 114 115 27 46 105 109 99 44
54 92 4 5 5 29 23 5
Product Information Sheet - NPT01
per
Well
96 well plate 2.5–3.0 x 104
24 well plate 1.25–1.5 x 105
12 well plate 2.5–3.0 x 105
6 well plate 5.0–6.0 x 105
60 mm 1.0–1.5 x 106
100 mm 2.5–3.0 x
Application Note - Universal ProbeLibrary Set: One Transcriptome - One Kit
re
sc
en
ce
(
64
0/
53
0)
Cycle number
0 5 10 15 20 25 30 35 40 45
HybProbe
106 copies
105 copies
104 copies
103 copies
102 copies
10 copies
1 copy
Data Sheet - C8205
Expiration Date January 2006
Storage 2-8°C; After opening, transfer contents to
small vial provided for your convenience.
Mattson Galaxy 3020 FT-IR
40 60 80 100 120 140 160 180 200
EX-CELL CD CHO Serum-Free Medium - 14361C
CHO medium
supplemented with 8 mM L-glutamine at a density of
5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks.
4. Incubate the cells at 37 C in a humidified incubator with
8 - 10% CO2.
Blood Agar Base, 110886
25923 103-105
≥≥≥≥ 70 ββββ
Streptococcus pyogenes ATCC 12344 103-105
≥≥≥≥ 70 ββββ +
Streptococcus agalactiae ATCC 13813 103-105
≥≥≥≥ 70 ----
Streptococcus pneumoniae ATCC 6301 103-105
≥≥≥≥
EX-CELL CD CHO Serum-Free Medium - 14360C
CHO medium
supplemented with 8 mM L-glutamine at a density of
5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks.
4. Incubate the cells at 37 C in a humidified incubator with
8 - 10% CO2.
Data Sheet - B8900 - Lot 119H88081
derivative
40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360
0
50000
100000
150000
200000
250000
300000
m/z
Abundance
42
51
77
Extended High Density Growth of CHO-K1 Cells
were maintained in an incubator in 250 mL Bellco
spinner fl asks using a seeding density of 2 x 105 cells/mL in
100 mL of EX-CELL 302. Impeller speed was set to
approximately 60 rpm.
• Daily
Product Information Sheet - VP002
Humidified CO2 incubator
(8% CO2, 37 °C)
Sterile pipettes
Type II biosafety cabinet
Cell counter
70% isopropanol
Sub-Culturing Procedure
1. Passage cells at 6 × 105 viable cells
per mL
TB332 RiboJuice siRNA Transfection Reagent
Format 24 12 6 35 60 100
Number of adherent cells (× 105) 0.2–0.8 0.5–1.0 1–3 1–3 5–10 15–25
Number of suspension cells (× 105) 2–4 4–8 10–20 10–20 28–56 80–160
14385C - Technical Bulletin
HEK293 Viral Vector Medium?
We recommend inoculating cultures at
5.0 105 viable cells/mL for 3-day cultures or
3.0 105 viable cells/mL for 4-day cultures.
6. What is the suggested cell
Product Information Sheet - SAE0203
Chem., 267(13),
9321-9325 (1992).
3. Pelengaris, S. et al., Curr. Opin. Genet. Dev.,
10(1), 100-105 (2000)
4. Jarvik, J.W., and Telmer, C.A., Annu. Rev. Genet.,
32, 601-618 (1998).
5. Woychik
Data Sheet - C1528 - Lot 114K8804
5001000150020002500300035004000
0
20
40
60
80
100
%
T
r
a
n
s
m
i
t
t
a
n
c
e
m/z
Abundance
42
55
82
105
122 155
182
198 272
303
Data Sheet - F5886 - Lot 12K8801
September 2006
Storage 2-8°C; After opening, transfer contents to
small vial provided for your convenience.
Mattson Galaxy 3020 FT-IR
70 eV Electron Ionization Mass Spectrum
40 60 80 100 120 140 160
112979 - Total Dietary Fiber
glass crucible with frit)
diameter 40 mm
porosity 40 - 90 μm (G2)
For preparation of the crucible, see “Determination procedure”, section 2
Drying cabinet (105 °C)
Desiccator with drying agent
Cocaine-d3 (C3547)
September 2006
Storage 2-8°C; After
opening, transfer
contents to small
vial provided for
your convenience.
Mattson Galaxy 3020 FT-IR
70 eV Electron Ionization Mass Spectrum
40 60 80 100 120 140 160
Data Sheet - B8900 - Lot 81K88021
Storage 2-8°C; After opening, transfer contents to
small vial provided for your convenience.
Mattson Galaxy 3020 FT-IR
70 eV Electron Ionization Mass Spectrum
40 60 80 100 120
Data Sheet - B8900 - Lot 81K8802
Storage 2-8°C; After opening, transfer contents to
small vial provided for your convenience.
Mattson Galaxy 3020 FT-IR
70 eV Electron Ionization Mass Spectrum
40 60 80 100 120
Product Information Sheet -SAE0201
Chem., 267(13),
9321-9325 (1992).
3. Pelengaris, S. et al., Curr. Opin. Genet. Dev.,
10(1), 100-105 (2000)
4. Jarvik, J.W., and Telmer, C.A., Annu. Rev. Genet.,
32, 601-618 (1998).
5. Woychik
EX-CELL NS0 Serum-Free Medium for NS0 Cells Chemically Defined
serum-supplemented medium
into EX-CELLTM NS0 (supplemented with 8 mM L-glutamine
as described previously) at a minimum seeding density of
2 x 105 cells/mL in shaker flasks.
2. Incubate the flasks at 37 C
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